Isolation of monodisperse nanodisc-reconstituted membrane proteins using free flow electrophoresis

TY - JOUR

T1 - Isolation of monodisperse nanodisc-reconstituted membrane proteins using free flow electrophoresis

AU - Justesen, Bo Højen

AU - Laursen, Tomas

AU - Weber, Gerhard

AU - Fuglsang, Anja Thoe

AU - Møller, Birger Lindberg

AU - Günther-Pomorski, Thomas

PY - 2013/4/2

Y1 - 2013/4/2

N2 - Free flow electrophoresis is used for rapid and high-recovery isolation of homogeneous preparations of functionally active membrane proteins inserted into nanodiscs. The approach enables isolation of integral and membrane anchored proteins and is also applicable following introduction of, e.g., fluorescent tags. Preparative separation of membrane protein loaded nanodiscs from empty nanodiscs and protein aggregates results in monodisperse nanodisc preparations ideal for structural and functional characterization using biophysical methods.

AB - Free flow electrophoresis is used for rapid and high-recovery isolation of homogeneous preparations of functionally active membrane proteins inserted into nanodiscs. The approach enables isolation of integral and membrane anchored proteins and is also applicable following introduction of, e.g., fluorescent tags. Preparative separation of membrane protein loaded nanodiscs from empty nanodiscs and protein aggregates results in monodisperse nanodisc preparations ideal for structural and functional characterization using biophysical methods.

UR - http://www.scopus.com/inward/record.url?scp=84875771594&partnerID=8YFLogxK

U2 - 10.1021/ac4000915

DO - 10.1021/ac4000915

M3 - Letter

AN - SCOPUS:84875771594

SN - 0003-2700

VL - 85

SP - 3497

EP - 3500

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 7

ER -