@inbook{f7a48bb806034e76b7fd4374efc08222,
title = "Isolation of cancer stem cells from squamous cell carcinoma",
abstract = "Different cancer stem cell (CSC) populations can be found in many types of cancer, including squamous cell carcinoma (SSC). Diverse reports showed that CSC play a crucial role in the relapse of different types of cancer. CSC sustains tumor growth due to their capacity to self-renew and their potential to initiate secondary tumors with metastatic cancer features. Therefore, the development of methods for the isolation of CSC is a key step to explore the mechanisms underlying CSC maintenance. In this chapter, we provide a method for isolating CSC from cutaneous SSC using immunofluorescence labeling to allow the specific purification of CSC by fluorescence-activated cell sorting (FACS). This method is based on the use of CSC membrane markers, allowing as well the isolation CSC from different mouse strains.",
keywords = "Cancer stem cells, FACS, Mouse models, Skin, Squamous cell carcinoma",
author = "Silvia Fontenete and Mirna Perez-Moreno",
year = "2019",
doi = "10.1007/7651_2018_162",
language = "English",
isbn = "978-1-4939-8869-3",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "407--414",
editor = "Kursad Turksen",
booktitle = "Skin Stem Cells",
address = "United States",
edition = "2. ed.",
}
