Abstract
Different cancer stem cell (CSC) populations can be found in many types of cancer, including squamous cell carcinoma (SSC). Diverse reports showed that CSC play a crucial role in the relapse of different types of cancer. CSC sustains tumor growth due to their capacity to self-renew and their potential to initiate secondary tumors with metastatic cancer features. Therefore, the development of methods for the isolation of CSC is a key step to explore the mechanisms underlying CSC maintenance. In this chapter, we provide a method for isolating CSC from cutaneous SSC using immunofluorescence labeling to allow the specific purification of CSC by fluorescence-activated cell sorting (FACS). This method is based on the use of CSC membrane markers, allowing as well the isolation CSC from different mouse strains.
| Originalsprog | Engelsk |
|---|---|
| Titel | Skin Stem Cells : Methods and Protocols |
| Redaktører | Kursad Turksen |
| Antal sider | 8 |
| Forlag | Humana Press |
| Publikationsdato | 2019 |
| Udgave | 2. ed. |
| Sider | 407-414 |
| ISBN (Trykt) | 978-1-4939-8869-3 |
| ISBN (Elektronisk) | 978-1-4939-8870-9 |
| DOI | |
| Status | Udgivet - 2019 |
| Navn | Methods in Molecular Biology |
|---|---|
| Vol/bind | 1879 |
| ISSN | 1064-3745 |
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