Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols

Stefania De Vitis; Giuseppina Matarise; Francesca Pardeo; Rossella Catalano; Natalia Malara; Valentina Trunzo; Rossana Tallerico; Francesco Gentile; Patrizio Candeloro; Maria Laura Coluccio; Alessandro Massaro; Giuseppe Viglietto; Ennio Carbone; Jörg P. Kutter; Gerardo Perozziello; Enzo Di Fabrizio

doi:10.1016/j.mee.2014.04.013

Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols

Stefania De Vitis, Giuseppina Matarise, Francesca Pardeo, Rossella Catalano, Natalia Malara, Valentina Trunzo, Rossana Tallerico, Francesco Gentile, Patrizio Candeloro, Maria Laura Coluccio, Alessandro Massaro, Giuseppe Viglietto, Ennio Carbone, Jörg P. Kutter, Gerardo Perozziello^*, Enzo Di Fabrizio

^*Corresponding author for this work

5 Citations (Scopus)

Abstract

The aim of this work is the development of a microfluidic immunosensor for the immobilization of cancer cells and their separation from healthy cells by using "in situ" microfluidic biofunctionalization protocols. These protocols allow to link antibodies on microfluidic device surfaces and can be used to study the interaction between cell membrane and biomolecules. Moreover they allow to perform analysis with high processing speed, small quantity of reagents and samples, short reaction times and low production costs. In this work the developed protocols were used in microfluidic devices for the isolation of cancer cells in heterogeneous blood samples by exploiting the binding of specific antibody to an adhesion protein (EpCAM), overexpressed on the tumor cell membranes. The presented biofunctionalization protocols can be performed right before running the experiment: this allows to have a flexible platform where biomolecules of interest can be linked on the device surface according to the user's needs.

Original language	English
Journal	Microelectronic Engineering
Volume	124
Pages (from-to)	76-80
Number of pages	5
ISSN	0167-9317
DOIs	https://doi.org/10.1016/j.mee.2014.04.013
Publication status	Published - 25 Jul 2014

Keywords

Biofunctionalization protocols
Cancer cell isolations
Cell sorting
Microfluidic assay

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.mee.2014.04.013

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De Vitis, S., Matarise, G., Pardeo, F., Catalano, R., Malara, N., Trunzo, V., Tallerico, R., Gentile, F., Candeloro, P., Coluccio, M. L., Massaro, A., Viglietto, G., Carbone, E., Kutter, J. P., Perozziello, G., & Di Fabrizio, E. (2014). Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols. Microelectronic Engineering, 124, 76-80. https://doi.org/10.1016/j.mee.2014.04.013

De Vitis, S, Matarise, G, Pardeo, F, Catalano, R, Malara, N, Trunzo, V, Tallerico, R, Gentile, F, Candeloro, P, Coluccio, ML, Massaro, A, Viglietto, G, Carbone, E, Kutter, JP, Perozziello, G & Di Fabrizio, E 2014, 'Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols', Microelectronic Engineering, vol. 124, pp. 76-80. https://doi.org/10.1016/j.mee.2014.04.013

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AU - Matarise, Giuseppina

AU - Pardeo, Francesca

AU - Catalano, Rossella

AU - Malara, Natalia

AU - Trunzo, Valentina

AU - Tallerico, Rossana

AU - Gentile, Francesco

AU - Candeloro, Patrizio

AU - Coluccio, Maria Laura

AU - Massaro, Alessandro

AU - Viglietto, Giuseppe

AU - Carbone, Ennio

AU - Kutter, Jörg P.

AU - Perozziello, Gerardo

AU - Di Fabrizio, Enzo

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N2 - The aim of this work is the development of a microfluidic immunosensor for the immobilization of cancer cells and their separation from healthy cells by using "in situ" microfluidic biofunctionalization protocols. These protocols allow to link antibodies on microfluidic device surfaces and can be used to study the interaction between cell membrane and biomolecules. Moreover they allow to perform analysis with high processing speed, small quantity of reagents and samples, short reaction times and low production costs. In this work the developed protocols were used in microfluidic devices for the isolation of cancer cells in heterogeneous blood samples by exploiting the binding of specific antibody to an adhesion protein (EpCAM), overexpressed on the tumor cell membranes. The presented biofunctionalization protocols can be performed right before running the experiment: this allows to have a flexible platform where biomolecules of interest can be linked on the device surface according to the user's needs.

AB - The aim of this work is the development of a microfluidic immunosensor for the immobilization of cancer cells and their separation from healthy cells by using "in situ" microfluidic biofunctionalization protocols. These protocols allow to link antibodies on microfluidic device surfaces and can be used to study the interaction between cell membrane and biomolecules. Moreover they allow to perform analysis with high processing speed, small quantity of reagents and samples, short reaction times and low production costs. In this work the developed protocols were used in microfluidic devices for the isolation of cancer cells in heterogeneous blood samples by exploiting the binding of specific antibody to an adhesion protein (EpCAM), overexpressed on the tumor cell membranes. The presented biofunctionalization protocols can be performed right before running the experiment: this allows to have a flexible platform where biomolecules of interest can be linked on the device surface according to the user's needs.

KW - Biofunctionalization protocols

KW - Cancer cell isolations

KW - Cell sorting

KW - Microfluidic assay

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VL - 124

SP - 76

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JO - Microelectronic Engineering

JF - Microelectronic Engineering

ER -

Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols

Abstract

Keywords

UN SDGs

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