Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols

Stefania De Vitis; Giuseppina Matarise; Francesca Pardeo; Rossella Catalano; Natalia Malara; Valentina Trunzo; Rossana Tallerico; Francesco Gentile; Patrizio Candeloro; Maria Laura Coluccio; Alessandro Massaro; Giuseppe Viglietto; Ennio Carbone; Jörg P. Kutter; Gerardo Perozziello; Enzo Di Fabrizio

doi:10.1016/j.mee.2014.04.013

Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols

Stefania De Vitis, Giuseppina Matarise, Francesca Pardeo, Rossella Catalano, Natalia Malara, Valentina Trunzo, Rossana Tallerico, Francesco Gentile, Patrizio Candeloro, Maria Laura Coluccio, Alessandro Massaro, Giuseppe Viglietto, Ennio Carbone, Jörg P. Kutter, Gerardo Perozziello^*, Enzo Di Fabrizio

^*Corresponding author af dette arbejde

5 Citationer (Scopus)

Abstract

The aim of this work is the development of a microfluidic immunosensor for the immobilization of cancer cells and their separation from healthy cells by using "in situ" microfluidic biofunctionalization protocols. These protocols allow to link antibodies on microfluidic device surfaces and can be used to study the interaction between cell membrane and biomolecules. Moreover they allow to perform analysis with high processing speed, small quantity of reagents and samples, short reaction times and low production costs. In this work the developed protocols were used in microfluidic devices for the isolation of cancer cells in heterogeneous blood samples by exploiting the binding of specific antibody to an adhesion protein (EpCAM), overexpressed on the tumor cell membranes. The presented biofunctionalization protocols can be performed right before running the experiment: this allows to have a flexible platform where biomolecules of interest can be linked on the device surface according to the user's needs.

Originalsprog	Engelsk
Tidsskrift	Microelectronic Engineering
Vol/bind	124
Sider (fra-til)	76-80
Antal sider	5
ISSN	0167-9317
DOI	https://doi.org/10.1016/j.mee.2014.04.013
Status	Udgivet - 25 jul. 2014

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De Vitis, S., Matarise, G., Pardeo, F., Catalano, R., Malara, N., Trunzo, V., Tallerico, R., Gentile, F., Candeloro, P., Coluccio, M. L., Massaro, A., Viglietto, G., Carbone, E., Kutter, J. P., Perozziello, G., & Di Fabrizio, E. (2014). Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols. Microelectronic Engineering, 124, 76-80. https://doi.org/10.1016/j.mee.2014.04.013

De Vitis, S, Matarise, G, Pardeo, F, Catalano, R, Malara, N, Trunzo, V, Tallerico, R, Gentile, F, Candeloro, P, Coluccio, ML, Massaro, A, Viglietto, G, Carbone, E, Kutter, JP, Perozziello, G & Di Fabrizio, E 2014, 'Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols', Microelectronic Engineering, bind 124, s. 76-80. https://doi.org/10.1016/j.mee.2014.04.013

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abstract = "The aim of this work is the development of a microfluidic immunosensor for the immobilization of cancer cells and their separation from healthy cells by using {"}in situ{"} microfluidic biofunctionalization protocols. These protocols allow to link antibodies on microfluidic device surfaces and can be used to study the interaction between cell membrane and biomolecules. Moreover they allow to perform analysis with high processing speed, small quantity of reagents and samples, short reaction times and low production costs. In this work the developed protocols were used in microfluidic devices for the isolation of cancer cells in heterogeneous blood samples by exploiting the binding of specific antibody to an adhesion protein (EpCAM), overexpressed on the tumor cell membranes. The presented biofunctionalization protocols can be performed right before running the experiment: this allows to have a flexible platform where biomolecules of interest can be linked on the device surface according to the user's needs.",

keywords = "Biofunctionalization protocols, Cancer cell isolations, Cell sorting, Microfluidic assay",

author = "{De Vitis}, Stefania and Giuseppina Matarise and Francesca Pardeo and Rossella Catalano and Natalia Malara and Valentina Trunzo and Rossana Tallerico and Francesco Gentile and Patrizio Candeloro and Coluccio, {Maria Laura} and Alessandro Massaro and Giuseppe Viglietto and Ennio Carbone and Kutter, {J{\"o}rg P.} and Gerardo Perozziello and {Di Fabrizio}, Enzo",

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doi = "10.1016/j.mee.2014.04.013",

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AU - De Vitis, Stefania

AU - Matarise, Giuseppina

AU - Pardeo, Francesca

AU - Catalano, Rossella

AU - Malara, Natalia

AU - Trunzo, Valentina

AU - Tallerico, Rossana

AU - Gentile, Francesco

AU - Candeloro, Patrizio

AU - Coluccio, Maria Laura

AU - Massaro, Alessandro

AU - Viglietto, Giuseppe

AU - Carbone, Ennio

AU - Kutter, Jörg P.

AU - Perozziello, Gerardo

AU - Di Fabrizio, Enzo

PY - 2014/7/25

Y1 - 2014/7/25

N2 - The aim of this work is the development of a microfluidic immunosensor for the immobilization of cancer cells and their separation from healthy cells by using "in situ" microfluidic biofunctionalization protocols. These protocols allow to link antibodies on microfluidic device surfaces and can be used to study the interaction between cell membrane and biomolecules. Moreover they allow to perform analysis with high processing speed, small quantity of reagents and samples, short reaction times and low production costs. In this work the developed protocols were used in microfluidic devices for the isolation of cancer cells in heterogeneous blood samples by exploiting the binding of specific antibody to an adhesion protein (EpCAM), overexpressed on the tumor cell membranes. The presented biofunctionalization protocols can be performed right before running the experiment: this allows to have a flexible platform where biomolecules of interest can be linked on the device surface according to the user's needs.

AB - The aim of this work is the development of a microfluidic immunosensor for the immobilization of cancer cells and their separation from healthy cells by using "in situ" microfluidic biofunctionalization protocols. These protocols allow to link antibodies on microfluidic device surfaces and can be used to study the interaction between cell membrane and biomolecules. Moreover they allow to perform analysis with high processing speed, small quantity of reagents and samples, short reaction times and low production costs. In this work the developed protocols were used in microfluidic devices for the isolation of cancer cells in heterogeneous blood samples by exploiting the binding of specific antibody to an adhesion protein (EpCAM), overexpressed on the tumor cell membranes. The presented biofunctionalization protocols can be performed right before running the experiment: this allows to have a flexible platform where biomolecules of interest can be linked on the device surface according to the user's needs.

KW - Biofunctionalization protocols

KW - Cancer cell isolations

KW - Cell sorting

KW - Microfluidic assay

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DO - 10.1016/j.mee.2014.04.013

M3 - Journal article

AN - SCOPUS:84901836503

SN - 0167-9317

VL - 124

SP - 76

EP - 80

JO - Microelectronic Engineering

JF - Microelectronic Engineering

ER -

Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols

Abstract

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