Isolation in vitro of Strongwellsea castrans [Fungi: Entomophthorales] a pathogen of adult cabbage root flies, Delia radicum [Dipt.: Anthomyiidae]

J. Eilenberg; N. Wilding; J. Bresciani

doi:10.1007/BF02372975

Isolation in vitro of Strongwellsea castrans [Fungi: Entomophthorales] a pathogen of adult cabbage root flies, Delia radicum [Dipt.: Anthomyiidae]

J. Eilenberg^*, N. Wilding, J. Bresciani

^*Corresponding author for this work

Section for Organismal Biology

8 Citations (Scopus)

Abstract

The entomogenous fungus Strongwellsea castrans was isolated in vitro for the first time, by incubating conidia projected from infected cabbage root flies (Delia radicum) in a simple, semi-defined liquid medium comprising dextrose, yeast extract and lactalbumin hydrolysate buffered to pH 7. The fungus grew as long unitunicate hyphae. After transfer to a solid nutrient medium, multinucleate hyphal bodies were formed which developed a thick, laminated wall. Neither conidia nor resting spores developed in liquid or on solid media and the fungus survived successive sub-culturing only in liquid media. Using the API-ZYM system, tests on extracts on hyphae of S. castrans were positive for 11 enzymes but there were no consistent differences in enzyme profiles between S. castrans and fungi of the related genus Erynia.

Original language	English
Journal	Entomophaga
Volume	37
Issue number	1
Pages (from-to)	65-77
Number of pages	13
ISSN	0013-8959
DOIs	https://doi.org/10.1007/BF02372975
Publication status	Published - 1 Mar 1992

Keywords

Delia radicum
entomogenous fungus
Entomophthorales
enzyme profile
in vitro culture
Strongwellsea castrans

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10.1007/BF02372975

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title = "Isolation in vitro of Strongwellsea castrans [Fungi: Entomophthorales] a pathogen of adult cabbage root flies, Delia radicum [Dipt.: Anthomyiidae]",

abstract = "The entomogenous fungus Strongwellsea castrans was isolated in vitro for the first time, by incubating conidia projected from infected cabbage root flies (Delia radicum) in a simple, semi-defined liquid medium comprising dextrose, yeast extract and lactalbumin hydrolysate buffered to pH 7. The fungus grew as long unitunicate hyphae. After transfer to a solid nutrient medium, multinucleate hyphal bodies were formed which developed a thick, laminated wall. Neither conidia nor resting spores developed in liquid or on solid media and the fungus survived successive sub-culturing only in liquid media. Using the API-ZYM system, tests on extracts on hyphae of S. castrans were positive for 11 enzymes but there were no consistent differences in enzyme profiles between S. castrans and fungi of the related genus Erynia.",

keywords = "Delia radicum, entomogenous fungus, Entomophthorales, enzyme profile, in vitro culture, Strongwellsea castrans",

author = "J. Eilenberg and N. Wilding and J. Bresciani",

year = "1992",

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doi = "10.1007/BF02372975",

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T1 - Isolation in vitro of Strongwellsea castrans [Fungi

T2 - Entomophthorales] a pathogen of adult cabbage root flies, Delia radicum [Dipt.: Anthomyiidae]

AU - Eilenberg, J.

AU - Wilding, N.

AU - Bresciani, J.

PY - 1992/3/1

Y1 - 1992/3/1

N2 - The entomogenous fungus Strongwellsea castrans was isolated in vitro for the first time, by incubating conidia projected from infected cabbage root flies (Delia radicum) in a simple, semi-defined liquid medium comprising dextrose, yeast extract and lactalbumin hydrolysate buffered to pH 7. The fungus grew as long unitunicate hyphae. After transfer to a solid nutrient medium, multinucleate hyphal bodies were formed which developed a thick, laminated wall. Neither conidia nor resting spores developed in liquid or on solid media and the fungus survived successive sub-culturing only in liquid media. Using the API-ZYM system, tests on extracts on hyphae of S. castrans were positive for 11 enzymes but there were no consistent differences in enzyme profiles between S. castrans and fungi of the related genus Erynia.

AB - The entomogenous fungus Strongwellsea castrans was isolated in vitro for the first time, by incubating conidia projected from infected cabbage root flies (Delia radicum) in a simple, semi-defined liquid medium comprising dextrose, yeast extract and lactalbumin hydrolysate buffered to pH 7. The fungus grew as long unitunicate hyphae. After transfer to a solid nutrient medium, multinucleate hyphal bodies were formed which developed a thick, laminated wall. Neither conidia nor resting spores developed in liquid or on solid media and the fungus survived successive sub-culturing only in liquid media. Using the API-ZYM system, tests on extracts on hyphae of S. castrans were positive for 11 enzymes but there were no consistent differences in enzyme profiles between S. castrans and fungi of the related genus Erynia.

KW - Delia radicum

KW - entomogenous fungus

KW - Entomophthorales

KW - enzyme profile

KW - in vitro culture

KW - Strongwellsea castrans

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Isolation in vitro of Strongwellsea castrans [Fungi: Entomophthorales] a pathogen of adult cabbage root flies, Delia radicum [Dipt.: Anthomyiidae]

Abstract

Keywords

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