Isolation in vitro of Strongwellsea castrans [Fungi: Entomophthorales] a pathogen of adult cabbage root flies, Delia radicum [Dipt.: Anthomyiidae]

J. Eilenberg; N. Wilding; J. Bresciani

doi:10.1007/BF02372975

Isolation in vitro of Strongwellsea castrans [Fungi: Entomophthorales] a pathogen of adult cabbage root flies, Delia radicum [Dipt.: Anthomyiidae]

J. Eilenberg^*, N. Wilding, J. Bresciani

^*Corresponding author af dette arbejde

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Abstract

The entomogenous fungus Strongwellsea castrans was isolated in vitro for the first time, by incubating conidia projected from infected cabbage root flies (Delia radicum) in a simple, semi-defined liquid medium comprising dextrose, yeast extract and lactalbumin hydrolysate buffered to pH 7. The fungus grew as long unitunicate hyphae. After transfer to a solid nutrient medium, multinucleate hyphal bodies were formed which developed a thick, laminated wall. Neither conidia nor resting spores developed in liquid or on solid media and the fungus survived successive sub-culturing only in liquid media. Using the API-ZYM system, tests on extracts on hyphae of S. castrans were positive for 11 enzymes but there were no consistent differences in enzyme profiles between S. castrans and fungi of the related genus Erynia.

Originalsprog	Engelsk
Tidsskrift	Entomophaga
Vol/bind	37
Udgave nummer	1
Sider (fra-til)	65-77
Antal sider	13
ISSN	0013-8959
DOI	https://doi.org/10.1007/BF02372975
Status	Udgivet - 1 mar. 1992

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title = "Isolation in vitro of Strongwellsea castrans [Fungi: Entomophthorales] a pathogen of adult cabbage root flies, Delia radicum [Dipt.: Anthomyiidae]",

abstract = "The entomogenous fungus Strongwellsea castrans was isolated in vitro for the first time, by incubating conidia projected from infected cabbage root flies (Delia radicum) in a simple, semi-defined liquid medium comprising dextrose, yeast extract and lactalbumin hydrolysate buffered to pH 7. The fungus grew as long unitunicate hyphae. After transfer to a solid nutrient medium, multinucleate hyphal bodies were formed which developed a thick, laminated wall. Neither conidia nor resting spores developed in liquid or on solid media and the fungus survived successive sub-culturing only in liquid media. Using the API-ZYM system, tests on extracts on hyphae of S. castrans were positive for 11 enzymes but there were no consistent differences in enzyme profiles between S. castrans and fungi of the related genus Erynia.",

keywords = "Delia radicum, entomogenous fungus, Entomophthorales, enzyme profile, in vitro culture, Strongwellsea castrans",

author = "J. Eilenberg and N. Wilding and J. Bresciani",

year = "1992",

month = mar,

day = "1",

doi = "10.1007/BF02372975",

language = "English",

volume = "37",

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journal = "BioControl",

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T1 - Isolation in vitro of Strongwellsea castrans [Fungi

T2 - Entomophthorales] a pathogen of adult cabbage root flies, Delia radicum [Dipt.: Anthomyiidae]

AU - Eilenberg, J.

AU - Wilding, N.

AU - Bresciani, J.

PY - 1992/3/1

Y1 - 1992/3/1

N2 - The entomogenous fungus Strongwellsea castrans was isolated in vitro for the first time, by incubating conidia projected from infected cabbage root flies (Delia radicum) in a simple, semi-defined liquid medium comprising dextrose, yeast extract and lactalbumin hydrolysate buffered to pH 7. The fungus grew as long unitunicate hyphae. After transfer to a solid nutrient medium, multinucleate hyphal bodies were formed which developed a thick, laminated wall. Neither conidia nor resting spores developed in liquid or on solid media and the fungus survived successive sub-culturing only in liquid media. Using the API-ZYM system, tests on extracts on hyphae of S. castrans were positive for 11 enzymes but there were no consistent differences in enzyme profiles between S. castrans and fungi of the related genus Erynia.

AB - The entomogenous fungus Strongwellsea castrans was isolated in vitro for the first time, by incubating conidia projected from infected cabbage root flies (Delia radicum) in a simple, semi-defined liquid medium comprising dextrose, yeast extract and lactalbumin hydrolysate buffered to pH 7. The fungus grew as long unitunicate hyphae. After transfer to a solid nutrient medium, multinucleate hyphal bodies were formed which developed a thick, laminated wall. Neither conidia nor resting spores developed in liquid or on solid media and the fungus survived successive sub-culturing only in liquid media. Using the API-ZYM system, tests on extracts on hyphae of S. castrans were positive for 11 enzymes but there were no consistent differences in enzyme profiles between S. castrans and fungi of the related genus Erynia.

KW - Delia radicum

KW - entomogenous fungus

KW - Entomophthorales

KW - enzyme profile

KW - in vitro culture

KW - Strongwellsea castrans

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U2 - 10.1007/BF02372975

DO - 10.1007/BF02372975

M3 - Journal article

AN - SCOPUS:0003247816

SN - 1386-6141

VL - 37

SP - 65

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JO - BioControl

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IS - 1

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Isolation in vitro of Strongwellsea castrans [Fungi: Entomophthorales] a pathogen of adult cabbage root flies, Delia radicum [Dipt.: Anthomyiidae]

Abstract

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