Schinus terebinthifolia leaf lectin (SteLL) has anti-infective action and modulates the response of Staphylococcus aureus-infected macrophages

Isana Maria de Souza Feitosa Lima; Adrielle Zagmignan; Deivid Martins Santos; Hermerson Sousa Maia; Lucas dos Santos Silva; Brenda da Silva Cutrim; Silvamara Leite Vieira; Clovis Macêdo Bezerra Filho; Eduardo Martins de Sousa; Thiago Henrique Napoleão; Karen Angeliki Krogfelt; Anders Løbner-Olesen; Patricía Maria Guedes Paiva; Luis Cláudio Nascimento da Silva

doi:10.1038/s41598-019-54616-x

Schinus terebinthifolia leaf lectin (SteLL) has anti-infective action and modulates the response of Staphylococcus aureus-infected macrophages

Isana Maria de Souza Feitosa Lima, Adrielle Zagmignan, Deivid Martins Santos, Hermerson Sousa Maia, Lucas dos Santos Silva, Brenda da Silva Cutrim, Silvamara Leite Vieira, Clovis Macêdo Bezerra Filho, Eduardo Martins de Sousa, Thiago Henrique Napoleão, Karen Angeliki Krogfelt, Anders Løbner-Olesen, Patricía Maria Guedes Paiva, Luis Cláudio Nascimento da Silva

Functional Genomics

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Abstract

Staphylococcus aureus is recognized as an important pathogen causing a wide spectrum of diseases. Here we examined the antimicrobial effects of the lectin isolated from leaves of Schinus terebinthifolia Raddi (SteLL) against S. aureus using in vitro assays and an infection model based on Galleria mellonella larvae. The actions of SteLL on mice macrophages and S. aureus-infected macrophages were also evaluated. SteLL at 16 µg/mL (8 × MIC) increased cell mass and DNA content of S. aureus in relation to untreated bacteria, suggesting that SteLL impairs cell division. Unlike ciprofloxacin, SteLL did not induce the expression of recA, crucial for DNA repair through SOS response. The antimicrobial action of SteLL was partially inhibited by 50 mM N-acetylglucosamine. SteLL reduced staphyloxathin production and increased ciprofloxacin activity towards S. aureus. This lectin also improved the survival of G. mellonella larvae infected with S. aureus. Furthermore, SteLL induced the release of cytokines (IL-6, IL-10, IL-17A, and TNF-α), nitric oxide and superoxide anion by macrophagens. The lectin improved the bactericidal action of macrophages towards S. aureus; while the expression of IL-17A and IFN-γ was downregulated in infected macrophages. These evidences suggest SteLL as important lead molecule in the development of anti-infective agents against S. aureus.

Original language	English
Article number	18159
Journal	Scientific Reports
Volume	9
Number of pages	14
ISSN	2045-2322
DOIs	https://doi.org/10.1038/s41598-019-54616-x
Publication status	Published - 1 Dec 2019

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Schinus terebinthifolia leaf lectin (SteLL) has anti-infective action and modulates the response of Staphylococcus aureus-infected macrophagesFinal published version, 1.64 MBLicence: CC BY

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Lima, I. M. D. S. F., Zagmignan, A., Santos, D. M., Maia, H. S., Silva, L. D. S., Cutrim, B. D. S., Vieira, S. L., Filho, C. M. B., de Sousa, E. M., Napoleão, T. H., Krogfelt, K. A., Løbner-Olesen, A., Paiva, P. M. G., & da Silva, L. C. N. (2019). Schinus terebinthifolia leaf lectin (SteLL) has anti-infective action and modulates the response of Staphylococcus aureus-infected macrophages. Scientific Reports, 9, Article 18159. https://doi.org/10.1038/s41598-019-54616-x

Schinus terebinthifolia leaf lectin (SteLL) has anti-infective action and modulates the response of Staphylococcus aureus-infected macrophages. / Lima, Isana Maria de Souza Feitosa; Zagmignan, Adrielle; Santos, Deivid Martins et al.
In: Scientific Reports, Vol. 9, 18159, 01.12.2019.

Research output: Contribution to journal › Journal article › Research › peer-review

Lima, IMDSF, Zagmignan, A, Santos, DM, Maia, HS, Silva, LDS, Cutrim, BDS, Vieira, SL, Filho, CMB, de Sousa, EM, Napoleão, TH, Krogfelt, KA, Løbner-Olesen, A, Paiva, PMG & da Silva, LCN 2019, 'Schinus terebinthifolia leaf lectin (SteLL) has anti-infective action and modulates the response of Staphylococcus aureus-infected macrophages', Scientific Reports, vol. 9, 18159. https://doi.org/10.1038/s41598-019-54616-x

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abstract = "Staphylococcus aureus is recognized as an important pathogen causing a wide spectrum of diseases. Here we examined the antimicrobial effects of the lectin isolated from leaves of Schinus terebinthifolia Raddi (SteLL) against S. aureus using in vitro assays and an infection model based on Galleria mellonella larvae. The actions of SteLL on mice macrophages and S. aureus-infected macrophages were also evaluated. SteLL at 16 µg/mL (8 × MIC) increased cell mass and DNA content of S. aureus in relation to untreated bacteria, suggesting that SteLL impairs cell division. Unlike ciprofloxacin, SteLL did not induce the expression of recA, crucial for DNA repair through SOS response. The antimicrobial action of SteLL was partially inhibited by 50 mM N-acetylglucosamine. SteLL reduced staphyloxathin production and increased ciprofloxacin activity towards S. aureus. This lectin also improved the survival of G. mellonella larvae infected with S. aureus. Furthermore, SteLL induced the release of cytokines (IL-6, IL-10, IL-17A, and TNF-α), nitric oxide and superoxide anion by macrophagens. The lectin improved the bactericidal action of macrophages towards S. aureus; while the expression of IL-17A and IFN-γ was downregulated in infected macrophages. These evidences suggest SteLL as important lead molecule in the development of anti-infective agents against S. aureus.",

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AU - Lima, Isana Maria de Souza Feitosa

AU - Zagmignan, Adrielle

AU - Santos, Deivid Martins

AU - Maia, Hermerson Sousa

AU - Silva, Lucas dos Santos

AU - Cutrim, Brenda da Silva

AU - Vieira, Silvamara Leite

AU - Filho, Clovis Macêdo Bezerra

AU - de Sousa, Eduardo Martins

AU - Napoleão, Thiago Henrique

AU - Krogfelt, Karen Angeliki

AU - Løbner-Olesen, Anders

AU - Paiva, Patricía Maria Guedes

AU - da Silva, Luis Cláudio Nascimento

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N2 - Staphylococcus aureus is recognized as an important pathogen causing a wide spectrum of diseases. Here we examined the antimicrobial effects of the lectin isolated from leaves of Schinus terebinthifolia Raddi (SteLL) against S. aureus using in vitro assays and an infection model based on Galleria mellonella larvae. The actions of SteLL on mice macrophages and S. aureus-infected macrophages were also evaluated. SteLL at 16 µg/mL (8 × MIC) increased cell mass and DNA content of S. aureus in relation to untreated bacteria, suggesting that SteLL impairs cell division. Unlike ciprofloxacin, SteLL did not induce the expression of recA, crucial for DNA repair through SOS response. The antimicrobial action of SteLL was partially inhibited by 50 mM N-acetylglucosamine. SteLL reduced staphyloxathin production and increased ciprofloxacin activity towards S. aureus. This lectin also improved the survival of G. mellonella larvae infected with S. aureus. Furthermore, SteLL induced the release of cytokines (IL-6, IL-10, IL-17A, and TNF-α), nitric oxide and superoxide anion by macrophagens. The lectin improved the bactericidal action of macrophages towards S. aureus; while the expression of IL-17A and IFN-γ was downregulated in infected macrophages. These evidences suggest SteLL as important lead molecule in the development of anti-infective agents against S. aureus.

AB - Staphylococcus aureus is recognized as an important pathogen causing a wide spectrum of diseases. Here we examined the antimicrobial effects of the lectin isolated from leaves of Schinus terebinthifolia Raddi (SteLL) against S. aureus using in vitro assays and an infection model based on Galleria mellonella larvae. The actions of SteLL on mice macrophages and S. aureus-infected macrophages were also evaluated. SteLL at 16 µg/mL (8 × MIC) increased cell mass and DNA content of S. aureus in relation to untreated bacteria, suggesting that SteLL impairs cell division. Unlike ciprofloxacin, SteLL did not induce the expression of recA, crucial for DNA repair through SOS response. The antimicrobial action of SteLL was partially inhibited by 50 mM N-acetylglucosamine. SteLL reduced staphyloxathin production and increased ciprofloxacin activity towards S. aureus. This lectin also improved the survival of G. mellonella larvae infected with S. aureus. Furthermore, SteLL induced the release of cytokines (IL-6, IL-10, IL-17A, and TNF-α), nitric oxide and superoxide anion by macrophagens. The lectin improved the bactericidal action of macrophages towards S. aureus; while the expression of IL-17A and IFN-γ was downregulated in infected macrophages. These evidences suggest SteLL as important lead molecule in the development of anti-infective agents against S. aureus.

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DO - 10.1038/s41598-019-54616-x

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JO - Scientific Reports

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M1 - 18159

ER -

Schinus terebinthifolia leaf lectin (SteLL) has anti-infective action and modulates the response of Staphylococcus aureus-infected macrophages

Abstract

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