Schinus terebinthifolia leaf lectin (SteLL) has anti-infective action and modulates the response of Staphylococcus aureus-infected macrophages

Isana Maria de Souza Feitosa Lima; Adrielle Zagmignan; Deivid Martins Santos; Hermerson Sousa Maia; Lucas dos Santos Silva; Brenda da Silva Cutrim; Silvamara Leite Vieira; Clovis Macêdo Bezerra Filho; Eduardo Martins de Sousa; Thiago Henrique Napoleão; Karen Angeliki Krogfelt; Anders Løbner-Olesen; Patricía Maria Guedes Paiva; Luis Cláudio Nascimento da Silva

doi:10.1038/s41598-019-54616-x

Schinus terebinthifolia leaf lectin (SteLL) has anti-infective action and modulates the response of Staphylococcus aureus-infected macrophages

Isana Maria de Souza Feitosa Lima, Adrielle Zagmignan, Deivid Martins Santos, Hermerson Sousa Maia, Lucas dos Santos Silva, Brenda da Silva Cutrim, Silvamara Leite Vieira, Clovis Macêdo Bezerra Filho, Eduardo Martins de Sousa, Thiago Henrique Napoleão, Karen Angeliki Krogfelt, Anders Løbner-Olesen, Patricía Maria Guedes Paiva, Luis Cláudio Nascimento da Silva

Functional genomics

3 Citationer (Scopus)

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Abstract

Staphylococcus aureus is recognized as an important pathogen causing a wide spectrum of diseases. Here we examined the antimicrobial effects of the lectin isolated from leaves of Schinus terebinthifolia Raddi (SteLL) against S. aureus using in vitro assays and an infection model based on Galleria mellonella larvae. The actions of SteLL on mice macrophages and S. aureus-infected macrophages were also evaluated. SteLL at 16 µg/mL (8 × MIC) increased cell mass and DNA content of S. aureus in relation to untreated bacteria, suggesting that SteLL impairs cell division. Unlike ciprofloxacin, SteLL did not induce the expression of recA, crucial for DNA repair through SOS response. The antimicrobial action of SteLL was partially inhibited by 50 mM N-acetylglucosamine. SteLL reduced staphyloxathin production and increased ciprofloxacin activity towards S. aureus. This lectin also improved the survival of G. mellonella larvae infected with S. aureus. Furthermore, SteLL induced the release of cytokines (IL-6, IL-10, IL-17A, and TNF-α), nitric oxide and superoxide anion by macrophagens. The lectin improved the bactericidal action of macrophages towards S. aureus; while the expression of IL-17A and IFN-γ was downregulated in infected macrophages. These evidences suggest SteLL as important lead molecule in the development of anti-infective agents against S. aureus.

Originalsprog	Engelsk
Artikelnummer	18159
Tidsskrift	Scientific Reports
Vol/bind	9
Antal sider	14
ISSN	2045-2322
DOI	https://doi.org/10.1038/s41598-019-54616-x
Status	Udgivet - 1 dec. 2019

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10.1038/s41598-019-54616-xLicens: CC BY

Schinus terebinthifolia leaf lectin (SteLL) has anti-infective action and modulates the response of Staphylococcus aureus-infected macrophagesForlagets udgivne version, 1,64 MBLicens: CC BY

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Lima, I. M. D. S. F., Zagmignan, A., Santos, D. M., Maia, H. S., Silva, L. D. S., Cutrim, B. D. S., Vieira, S. L., Filho, C. M. B., de Sousa, E. M., Napoleão, T. H., Krogfelt, K. A., Løbner-Olesen, A., Paiva, P. M. G., & da Silva, L. C. N. (2019). Schinus terebinthifolia leaf lectin (SteLL) has anti-infective action and modulates the response of Staphylococcus aureus-infected macrophages. Scientific Reports, 9, [18159]. https://doi.org/10.1038/s41598-019-54616-x

Lima, IMDSF, Zagmignan, A, Santos, DM, Maia, HS, Silva, LDS, Cutrim, BDS, Vieira, SL, Filho, CMB, de Sousa, EM, Napoleão, TH, Krogfelt, KA, Løbner-Olesen, A, Paiva, PMG & da Silva, LCN 2019, 'Schinus terebinthifolia leaf lectin (SteLL) has anti-infective action and modulates the response of Staphylococcus aureus-infected macrophages', Scientific Reports, bind 9, 18159. https://doi.org/10.1038/s41598-019-54616-x

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title = "Schinus terebinthifolia leaf lectin (SteLL) has anti-infective action and modulates the response of Staphylococcus aureus-infected macrophages",

abstract = "Staphylococcus aureus is recognized as an important pathogen causing a wide spectrum of diseases. Here we examined the antimicrobial effects of the lectin isolated from leaves of Schinus terebinthifolia Raddi (SteLL) against S. aureus using in vitro assays and an infection model based on Galleria mellonella larvae. The actions of SteLL on mice macrophages and S. aureus-infected macrophages were also evaluated. SteLL at 16 µg/mL (8 × MIC) increased cell mass and DNA content of S. aureus in relation to untreated bacteria, suggesting that SteLL impairs cell division. Unlike ciprofloxacin, SteLL did not induce the expression of recA, crucial for DNA repair through SOS response. The antimicrobial action of SteLL was partially inhibited by 50 mM N-acetylglucosamine. SteLL reduced staphyloxathin production and increased ciprofloxacin activity towards S. aureus. This lectin also improved the survival of G. mellonella larvae infected with S. aureus. Furthermore, SteLL induced the release of cytokines (IL-6, IL-10, IL-17A, and TNF-α), nitric oxide and superoxide anion by macrophagens. The lectin improved the bactericidal action of macrophages towards S. aureus; while the expression of IL-17A and IFN-γ was downregulated in infected macrophages. These evidences suggest SteLL as important lead molecule in the development of anti-infective agents against S. aureus.",

author = "Lima, {Isana Maria de Souza Feitosa} and Adrielle Zagmignan and Santos, {Deivid Martins} and Maia, {Hermerson Sousa} and Silva, {Lucas dos Santos} and Cutrim, {Brenda da Silva} and Vieira, {Silvamara Leite} and Filho, {Clovis Mac{\^e}do Bezerra} and {de Sousa}, {Eduardo Martins} and Napole{\~a}o, {Thiago Henrique} and Krogfelt, {Karen Angeliki} and Anders L{\o}bner-Olesen and Paiva, {Patric{\'i}a Maria Guedes} and {da Silva}, {Luis Cl{\'a}udio Nascimento}",

year = "2019",

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doi = "10.1038/s41598-019-54616-x",

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T1 - Schinus terebinthifolia leaf lectin (SteLL) has anti-infective action and modulates the response of Staphylococcus aureus-infected macrophages

AU - Lima, Isana Maria de Souza Feitosa

AU - Zagmignan, Adrielle

AU - Santos, Deivid Martins

AU - Maia, Hermerson Sousa

AU - Silva, Lucas dos Santos

AU - Cutrim, Brenda da Silva

AU - Vieira, Silvamara Leite

AU - Filho, Clovis Macêdo Bezerra

AU - de Sousa, Eduardo Martins

AU - Napoleão, Thiago Henrique

AU - Krogfelt, Karen Angeliki

AU - Løbner-Olesen, Anders

AU - Paiva, Patricía Maria Guedes

AU - da Silva, Luis Cláudio Nascimento

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Staphylococcus aureus is recognized as an important pathogen causing a wide spectrum of diseases. Here we examined the antimicrobial effects of the lectin isolated from leaves of Schinus terebinthifolia Raddi (SteLL) against S. aureus using in vitro assays and an infection model based on Galleria mellonella larvae. The actions of SteLL on mice macrophages and S. aureus-infected macrophages were also evaluated. SteLL at 16 µg/mL (8 × MIC) increased cell mass and DNA content of S. aureus in relation to untreated bacteria, suggesting that SteLL impairs cell division. Unlike ciprofloxacin, SteLL did not induce the expression of recA, crucial for DNA repair through SOS response. The antimicrobial action of SteLL was partially inhibited by 50 mM N-acetylglucosamine. SteLL reduced staphyloxathin production and increased ciprofloxacin activity towards S. aureus. This lectin also improved the survival of G. mellonella larvae infected with S. aureus. Furthermore, SteLL induced the release of cytokines (IL-6, IL-10, IL-17A, and TNF-α), nitric oxide and superoxide anion by macrophagens. The lectin improved the bactericidal action of macrophages towards S. aureus; while the expression of IL-17A and IFN-γ was downregulated in infected macrophages. These evidences suggest SteLL as important lead molecule in the development of anti-infective agents against S. aureus.

AB - Staphylococcus aureus is recognized as an important pathogen causing a wide spectrum of diseases. Here we examined the antimicrobial effects of the lectin isolated from leaves of Schinus terebinthifolia Raddi (SteLL) against S. aureus using in vitro assays and an infection model based on Galleria mellonella larvae. The actions of SteLL on mice macrophages and S. aureus-infected macrophages were also evaluated. SteLL at 16 µg/mL (8 × MIC) increased cell mass and DNA content of S. aureus in relation to untreated bacteria, suggesting that SteLL impairs cell division. Unlike ciprofloxacin, SteLL did not induce the expression of recA, crucial for DNA repair through SOS response. The antimicrobial action of SteLL was partially inhibited by 50 mM N-acetylglucosamine. SteLL reduced staphyloxathin production and increased ciprofloxacin activity towards S. aureus. This lectin also improved the survival of G. mellonella larvae infected with S. aureus. Furthermore, SteLL induced the release of cytokines (IL-6, IL-10, IL-17A, and TNF-α), nitric oxide and superoxide anion by macrophagens. The lectin improved the bactericidal action of macrophages towards S. aureus; while the expression of IL-17A and IFN-γ was downregulated in infected macrophages. These evidences suggest SteLL as important lead molecule in the development of anti-infective agents against S. aureus.

U2 - 10.1038/s41598-019-54616-x

DO - 10.1038/s41598-019-54616-x

M3 - Journal article

C2 - 31796807

SN - 2045-2322

VL - 9

JO - Scientific Reports

JF - Scientific Reports

M1 - 18159

ER -

Schinus terebinthifolia leaf lectin (SteLL) has anti-infective action and modulates the response of Staphylococcus aureus-infected macrophages

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