Investigation of tissue-specific human orthologous alternative splice events in pig

Ann-Britt Nygaard Hillig; Claus Bøttcher Jørgensen; Susanna Cirera Salicio; Merete Fredholm

doi:10.1080/10495398.2010.497729

Investigation of tissue-specific human orthologous alternative splice events in pig

Ann-Britt Nygaard Hillig, Claus Bøttcher Jørgensen, Susanna Cirera Salicio, Merete Fredholm

5 Citations (Scopus)

Abstract

Alternative splicing of pre-mRNA can contribute to differences between tissues or cells either by regulating gene expression or creating proteins with various functions encoded by one gene. The number of investigated alternative splice events in pig has so far been limited. In this study we have investigated alternative splice events detected in humans, in orthologous pig genes. A total of 17 genes with predicted exon skipping events were selected for further studies. The splice events for the selected genes were experimentally verified using real-time quantitative PCR analysis (qPCR) with splice-specific primers in 19 different tissues. The same splice variants as reported in humans were detected in 15 orthologous pig genes, however, the expression pattern predicted in the in silico analyses was only experimentally verified in a few cases. The results support the findings that splice events resulting in preservation of open reading frame are indicative of a functional significance of the splice variants of the gene.

Original language	English
Journal	Animal Biotechnology (Print Edition)
Volume	21
Issue number	4
Pages (from-to)	203-216
Number of pages	14
ISSN	1049-5398
DOIs	https://doi.org/10.1080/10495398.2010.497729
Publication status	Published - Oct 2010

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title = "Investigation of tissue-specific human orthologous alternative splice events in pig",

abstract = "Alternative splicing of pre-mRNA can contribute to differences between tissues or cells either by regulating gene expression or creating proteins with various functions encoded by one gene. The number of investigated alternative splice events in pig has so far been limited. In this study we have investigated alternative splice events detected in humans, in orthologous pig genes. A total of 17 genes with predicted exon skipping events were selected for further studies. The splice events for the selected genes were experimentally verified using real-time quantitative PCR analysis (qPCR) with splice-specific primers in 19 different tissues. The same splice variants as reported in humans were detected in 15 orthologous pig genes, however, the expression pattern predicted in the in silico analyses was only experimentally verified in a few cases. The results support the findings that splice events resulting in preservation of open reading frame are indicative of a functional significance of the splice variants of the gene.",

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T1 - Investigation of tissue-specific human orthologous alternative splice events in pig

AU - Hillig, Ann-Britt Nygaard

AU - Jørgensen, Claus Bøttcher

AU - Salicio, Susanna Cirera

AU - Fredholm, Merete

PY - 2010/10

Y1 - 2010/10

N2 - Alternative splicing of pre-mRNA can contribute to differences between tissues or cells either by regulating gene expression or creating proteins with various functions encoded by one gene. The number of investigated alternative splice events in pig has so far been limited. In this study we have investigated alternative splice events detected in humans, in orthologous pig genes. A total of 17 genes with predicted exon skipping events were selected for further studies. The splice events for the selected genes were experimentally verified using real-time quantitative PCR analysis (qPCR) with splice-specific primers in 19 different tissues. The same splice variants as reported in humans were detected in 15 orthologous pig genes, however, the expression pattern predicted in the in silico analyses was only experimentally verified in a few cases. The results support the findings that splice events resulting in preservation of open reading frame are indicative of a functional significance of the splice variants of the gene.

AB - Alternative splicing of pre-mRNA can contribute to differences between tissues or cells either by regulating gene expression or creating proteins with various functions encoded by one gene. The number of investigated alternative splice events in pig has so far been limited. In this study we have investigated alternative splice events detected in humans, in orthologous pig genes. A total of 17 genes with predicted exon skipping events were selected for further studies. The splice events for the selected genes were experimentally verified using real-time quantitative PCR analysis (qPCR) with splice-specific primers in 19 different tissues. The same splice variants as reported in humans were detected in 15 orthologous pig genes, however, the expression pattern predicted in the in silico analyses was only experimentally verified in a few cases. The results support the findings that splice events resulting in preservation of open reading frame are indicative of a functional significance of the splice variants of the gene.

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DO - 10.1080/10495398.2010.497729

M3 - Journal article

C2 - 20967640

SN - 1049-5398

VL - 21

SP - 203

EP - 216

JO - Animal Biotechnology

JF - Animal Biotechnology

IS - 4

ER -

Investigation of tissue-specific human orthologous alternative splice events in pig

Abstract

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