TY - JOUR
T1 - Investigation of tissue-specific human orthologous alternative splice events in pig
AU - Hillig, Ann-Britt Nygaard
AU - Jørgensen, Claus Bøttcher
AU - Salicio, Susanna Cirera
AU - Fredholm, Merete
PY - 2010/10
Y1 - 2010/10
N2 - Alternative splicing of pre-mRNA can contribute to differences between tissues or cells either by regulating gene expression or creating proteins with various functions encoded by one gene. The number of investigated alternative splice events in pig has so far been limited. In this study we have investigated alternative splice events detected in humans, in orthologous pig genes. A total of 17 genes with predicted exon skipping events were selected for further studies. The splice events for the selected genes were experimentally verified using real-time quantitative PCR analysis (qPCR) with splice-specific primers in 19 different tissues. The same splice variants as reported in humans were detected in 15 orthologous pig genes, however, the expression pattern predicted in the in silico analyses was only experimentally verified in a few cases. The results support the findings that splice events resulting in preservation of open reading frame are indicative of a functional significance of the splice variants of the gene.
AB - Alternative splicing of pre-mRNA can contribute to differences between tissues or cells either by regulating gene expression or creating proteins with various functions encoded by one gene. The number of investigated alternative splice events in pig has so far been limited. In this study we have investigated alternative splice events detected in humans, in orthologous pig genes. A total of 17 genes with predicted exon skipping events were selected for further studies. The splice events for the selected genes were experimentally verified using real-time quantitative PCR analysis (qPCR) with splice-specific primers in 19 different tissues. The same splice variants as reported in humans were detected in 15 orthologous pig genes, however, the expression pattern predicted in the in silico analyses was only experimentally verified in a few cases. The results support the findings that splice events resulting in preservation of open reading frame are indicative of a functional significance of the splice variants of the gene.
U2 - 10.1080/10495398.2010.497729
DO - 10.1080/10495398.2010.497729
M3 - Journal article
C2 - 20967640
SN - 1049-5398
VL - 21
SP - 203
EP - 216
JO - Animal Biotechnology (Print Edition)
JF - Animal Biotechnology (Print Edition)
IS - 4
ER -