Investigation of the relationship between lactococcal host cell wall polysaccharide genotype and 936 phage receptor binding protein phylogeny

Jennifer Mahony; Witold Piotr Kot; James Murphy; Stuart Ainsworth; Horst Neve; Lars H. Hansen; Knut J. Heller; Søren Johannes Sørensen; Karin Hammer; Christian Cambillau; Finn Kvist Vogensen; Douwe van Sinderen

doi:10.1128/AEM.00653-13

Investigation of the relationship between lactococcal host cell wall polysaccharide genotype and 936 phage receptor binding protein phylogeny

Jennifer Mahony, Witold Piotr Kot, James Murphy, Stuart Ainsworth, Horst Neve, Lars H. Hansen, Knut J. Heller, Søren Johannes Sørensen, Karin Hammer, Christian Cambillau, Finn Kvist Vogensen, Douwe van Sinderen

69 Citations (Scopus)

Abstract

Comparative genomics of 11 lactococcal 936-type phages combined with host range analysis allowed subgrouping of these phage genomes, particularly with respect to their encoded receptor binding proteins. the so-called pellicle or cell wall polysaccharide of lactococcus lactis, which has been implicated as a host receptor of (certain) 936-type phages, is specified by a large gene cluster, which, among different lactococcal strains, contains highly conserved regions as well as regions of diversity. the regions of diversity within this cluster on the genomes of lactococcal strains mg1363, sk11, il1403, kf147, cv56, and uc509.9 were used for the development of a multiplex pcr system to identify the pellicle genotype of lactococcal strains used in this study. the resulting comparative analysis revealed an apparent correlation between the pellicle genotype of a given host strain and the host range of tested 936-type phages. such a correlation would allow prediction of the intrinsic 936-type phage sensitivity of a particular lactococcal strain and substantiates the notion that the lactococcal pellicle polysaccharide represents the receptor for (certain) 936-type phages while also partially explaining the molecular reasons behind the observed narrow host range of such phages.

Original language	English
Journal	Applied and Environmental Microbiology
Volume	79
Issue number	14
Pages (from-to)	4385-4392
Number of pages	8
ISSN	0099-2240
DOIs	https://doi.org/10.1128/AEM.00653-13
Publication status	Published - Jul 2013

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Mahony, J., Kot, W. P., Murphy, J., Ainsworth, S., Neve, H., Hansen, L. H., Heller, K. J., Sørensen, S. J., Hammer, K., Cambillau, C., Vogensen, F. K., & Sinderen, D. V. (2013). Investigation of the relationship between lactococcal host cell wall polysaccharide genotype and 936 phage receptor binding protein phylogeny. Applied and Environmental Microbiology, 79(14), 4385-4392. https://doi.org/10.1128/AEM.00653-13

Investigation of the relationship between lactococcal host cell wall polysaccharide genotype and 936 phage receptor binding protein phylogeny. / Mahony, Jennifer; Kot, Witold Piotr; Murphy, James et al.

In: Applied and Environmental Microbiology, Vol. 79, No. 14, 07.2013, p. 4385-4392.

Research output: Contribution to journal › Journal article › Research › peer-review

Mahony, J, Kot, WP, Murphy, J, Ainsworth, S, Neve, H, Hansen, LH, Heller, KJ, Sørensen, SJ, Hammer, K, Cambillau, C, Vogensen, FK & Sinderen, DV 2013, 'Investigation of the relationship between lactococcal host cell wall polysaccharide genotype and 936 phage receptor binding protein phylogeny', Applied and Environmental Microbiology, vol. 79, no. 14, pp. 4385-4392. https://doi.org/10.1128/AEM.00653-13

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title = "Investigation of the relationship between lactococcal host cell wall polysaccharide genotype and 936 phage receptor binding protein phylogeny",

abstract = "Comparative genomics of 11 lactococcal 936-type phages combined with host range analysis allowed subgrouping of these phage genomes, particularly with respect to their encoded receptor binding proteins. the so-called pellicle or cell wall polysaccharide of lactococcus lactis, which has been implicated as a host receptor of (certain) 936-type phages, is specified by a large gene cluster, which, among different lactococcal strains, contains highly conserved regions as well as regions of diversity. the regions of diversity within this cluster on the genomes of lactococcal strains mg1363, sk11, il1403, kf147, cv56, and uc509.9 were used for the development of a multiplex pcr system to identify the pellicle genotype of lactococcal strains used in this study. the resulting comparative analysis revealed an apparent correlation between the pellicle genotype of a given host strain and the host range of tested 936-type phages. such a correlation would allow prediction of the intrinsic 936-type phage sensitivity of a particular lactococcal strain and substantiates the notion that the lactococcal pellicle polysaccharide represents the receptor for (certain) 936-type phages while also partially explaining the molecular reasons behind the observed narrow host range of such phages.",

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AU - Neve, Horst

AU - Hansen, Lars H.

AU - Heller, Knut J.

AU - Sørensen, Søren Johannes

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AU - Vogensen, Finn Kvist

AU - Sinderen, Douwe van

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N2 - Comparative genomics of 11 lactococcal 936-type phages combined with host range analysis allowed subgrouping of these phage genomes, particularly with respect to their encoded receptor binding proteins. the so-called pellicle or cell wall polysaccharide of lactococcus lactis, which has been implicated as a host receptor of (certain) 936-type phages, is specified by a large gene cluster, which, among different lactococcal strains, contains highly conserved regions as well as regions of diversity. the regions of diversity within this cluster on the genomes of lactococcal strains mg1363, sk11, il1403, kf147, cv56, and uc509.9 were used for the development of a multiplex pcr system to identify the pellicle genotype of lactococcal strains used in this study. the resulting comparative analysis revealed an apparent correlation between the pellicle genotype of a given host strain and the host range of tested 936-type phages. such a correlation would allow prediction of the intrinsic 936-type phage sensitivity of a particular lactococcal strain and substantiates the notion that the lactococcal pellicle polysaccharide represents the receptor for (certain) 936-type phages while also partially explaining the molecular reasons behind the observed narrow host range of such phages.

AB - Comparative genomics of 11 lactococcal 936-type phages combined with host range analysis allowed subgrouping of these phage genomes, particularly with respect to their encoded receptor binding proteins. the so-called pellicle or cell wall polysaccharide of lactococcus lactis, which has been implicated as a host receptor of (certain) 936-type phages, is specified by a large gene cluster, which, among different lactococcal strains, contains highly conserved regions as well as regions of diversity. the regions of diversity within this cluster on the genomes of lactococcal strains mg1363, sk11, il1403, kf147, cv56, and uc509.9 were used for the development of a multiplex pcr system to identify the pellicle genotype of lactococcal strains used in this study. the resulting comparative analysis revealed an apparent correlation between the pellicle genotype of a given host strain and the host range of tested 936-type phages. such a correlation would allow prediction of the intrinsic 936-type phage sensitivity of a particular lactococcal strain and substantiates the notion that the lactococcal pellicle polysaccharide represents the receptor for (certain) 936-type phages while also partially explaining the molecular reasons behind the observed narrow host range of such phages.

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DO - 10.1128/AEM.00653-13

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EP - 4392

JO - Applied and Environmental Microbiology

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Investigation of the relationship between lactococcal host cell wall polysaccharide genotype and 936 phage receptor binding protein phylogeny

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