Intestinal levels of anandamide and oleoylethanolamide in food-deprived rats are regulated through their precursors

Gitte Petersen, Camilla Sørensen, Patricia C Schmid, Andreas Artmann, Mads Tang-Christensen, Steen H Hansen, Philip Just Larsen, Harald H O Schmid, Harald S. Hansen

    71 Citations (Scopus)

    Abstract

    The anorectic lipid oleoylethanolamide and the orexigenic lipid anandamide both belong to the group of N-acylethanolamines that are generated by the enzyme N-acylphosphatidylethanolamine-hydrolyzing phospholipase D. The levels of the two bioactive lipids were investigated in rat intestines after 24 h of starvation as well as after 1 and 4 h of re-feeding. Total levels of precursor phospholipids and N-acylethanolamines were decreased upon food-deprivation whereas the level of the anandamide precursor molecule was significantly increased. The level of 2-arachidonoyl-glycerol was unchanged as was the activity of N-acyltransferase, N-acylphosphatidylethanolamine-hydrolyzing phospholipase D, and fatty acid amide hydrolase upon starvation and re-feeding. It is concluded that remodeling of the amide-linked fatty acids of N-acylphosphatidylethanolamine is responsible for the opposite effects on levels of anandamide and oleoylethanolamide in intestines of food-deprived rats and not an alternative biochemical route for anandamide synthesis. Furthermore, linoleoylethanolamide, which accounted for more than 50 mol% of the endogenous pool of N-acylethanolamines, was found not to have the same inhibitory effect on food intake, as did oleoylethanolamide following oral administration.
    Original languageEnglish
    JournalBBA General Subjects
    Volume1761
    Issue number2
    Pages (from-to)143-50; discussion 141-2
    ISSN0304-4165
    DOIs
    Publication statusPublished - Feb 2006

    Keywords

    • Animals
    • Arachidonic Acids
    • Eating
    • Endocannabinoids
    • Food Deprivation
    • Intestines
    • Male
    • Oleic Acids
    • Phospholipids
    • Polyunsaturated Alkamides
    • Rats
    • Rats, Sprague-Dawley
    • Former Faculty of Pharmaceutical Sciences

    Fingerprint

    Dive into the research topics of 'Intestinal levels of anandamide and oleoylethanolamide in food-deprived rats are regulated through their precursors'. Together they form a unique fingerprint.

    Cite this