Intestinal levels of anandamide and oleoylethanolamide in food-deprived rats are regulated through their precursors

TY - JOUR

T1 - Intestinal levels of anandamide and oleoylethanolamide in food-deprived rats are regulated through their precursors

AU - Petersen, Gitte

AU - Sørensen, Camilla

AU - Schmid, Patricia C

AU - Artmann, Andreas

AU - Tang-Christensen, Mads

AU - Hansen, Steen H

AU - Larsen, Philip Just

AU - Schmid, Harald H O

AU - Hansen, Harald S.

PY - 2006/2

Y1 - 2006/2

N2 - The anorectic lipid oleoylethanolamide and the orexigenic lipid anandamide both belong to the group of N-acylethanolamines that are generated by the enzyme N-acylphosphatidylethanolamine-hydrolyzing phospholipase D. The levels of the two bioactive lipids were investigated in rat intestines after 24 h of starvation as well as after 1 and 4 h of re-feeding. Total levels of precursor phospholipids and N-acylethanolamines were decreased upon food-deprivation whereas the level of the anandamide precursor molecule was significantly increased. The level of 2-arachidonoyl-glycerol was unchanged as was the activity of N-acyltransferase, N-acylphosphatidylethanolamine-hydrolyzing phospholipase D, and fatty acid amide hydrolase upon starvation and re-feeding. It is concluded that remodeling of the amide-linked fatty acids of N-acylphosphatidylethanolamine is responsible for the opposite effects on levels of anandamide and oleoylethanolamide in intestines of food-deprived rats and not an alternative biochemical route for anandamide synthesis. Furthermore, linoleoylethanolamide, which accounted for more than 50 mol% of the endogenous pool of N-acylethanolamines, was found not to have the same inhibitory effect on food intake, as did oleoylethanolamide following oral administration.

AB - The anorectic lipid oleoylethanolamide and the orexigenic lipid anandamide both belong to the group of N-acylethanolamines that are generated by the enzyme N-acylphosphatidylethanolamine-hydrolyzing phospholipase D. The levels of the two bioactive lipids were investigated in rat intestines after 24 h of starvation as well as after 1 and 4 h of re-feeding. Total levels of precursor phospholipids and N-acylethanolamines were decreased upon food-deprivation whereas the level of the anandamide precursor molecule was significantly increased. The level of 2-arachidonoyl-glycerol was unchanged as was the activity of N-acyltransferase, N-acylphosphatidylethanolamine-hydrolyzing phospholipase D, and fatty acid amide hydrolase upon starvation and re-feeding. It is concluded that remodeling of the amide-linked fatty acids of N-acylphosphatidylethanolamine is responsible for the opposite effects on levels of anandamide and oleoylethanolamide in intestines of food-deprived rats and not an alternative biochemical route for anandamide synthesis. Furthermore, linoleoylethanolamide, which accounted for more than 50 mol% of the endogenous pool of N-acylethanolamines, was found not to have the same inhibitory effect on food intake, as did oleoylethanolamide following oral administration.

KW - Animals

KW - Arachidonic Acids

KW - Eating

KW - Endocannabinoids

KW - Food Deprivation

KW - Intestines

KW - Male

KW - Oleic Acids

KW - Phospholipids

KW - Polyunsaturated Alkamides

KW - Rats

KW - Rats, Sprague-Dawley

KW - Appetite Regulation

KW - Former Faculty of Pharmaceutical Sciences

U2 - 10.1016/j.bbalip.2005.12.011

DO - 10.1016/j.bbalip.2005.12.011

M3 - Journal article

C2 - 16478679

SN - 0304-4165

VL - 1761

SP - 143-50; discussion 141-2

JO - BBA General Subjects

JF - BBA General Subjects

IS - 2

ER -