TY - JOUR
T1 - Interferon-alpha induces transient suppressors of cytokine signalling expression in human T cells
AU - Brender, C
AU - Nielsen, M
AU - Röpke, C
AU - Nissen, M H
AU - Svejgaard, A
AU - Billestrup, N
AU - Geisler, C
AU - Ødum, N
N1 - Keywords: CD4-Positive T-Lymphocytes; Carrier Proteins; Cell Line; DNA-Binding Proteins; Gene Expression; Humans; Interferon-alpha; Interleukin-2; Intracellular Signaling Peptides and Proteins; Proteins; Repressor Proteins; Signal Transduction; Suppressor of Cytokine Signaling Proteins; Trans-Activators; Transcription Factors
PY - 2001
Y1 - 2001
N2 - The suppressors of cytokine signalling (SOCS) proteins comprise a newly identified family of negative feedback regulators of cytokine signalling. SOCS expression is differentially induced upon cytokine stimulation in different cell types. Here we show that interferon-alpha (IFNalpha) is a potent inducer of SOCS expression in human T cells, as high expression of CIS, SOCS-1, SOCS-2, and SOCS-3 was detectable after IFNalpha stimulation. After 4 h of stimulation, CIS, SOCS-1, and SOCS-3 expression had returned to baseline levels, whereas SOCS-2 expression had not declined. In contrast, after IL-2 induction neither CIS, SOCS-1, nor SOCS-2 expression levels declined after 6 h. In conclusion, we provide the first evidence that IFNalpha induces SOCS expression in human T cells. Moreover, we show that IFNalpha and IL-2 induce distinct patterns of expression kinetics, suggesting that dynamic changes in cytokine sensitivity might be mediated via induction of SOCS expression with different kinetics in T cells.
AB - The suppressors of cytokine signalling (SOCS) proteins comprise a newly identified family of negative feedback regulators of cytokine signalling. SOCS expression is differentially induced upon cytokine stimulation in different cell types. Here we show that interferon-alpha (IFNalpha) is a potent inducer of SOCS expression in human T cells, as high expression of CIS, SOCS-1, SOCS-2, and SOCS-3 was detectable after IFNalpha stimulation. After 4 h of stimulation, CIS, SOCS-1, and SOCS-3 expression had returned to baseline levels, whereas SOCS-2 expression had not declined. In contrast, after IL-2 induction neither CIS, SOCS-1, nor SOCS-2 expression levels declined after 6 h. In conclusion, we provide the first evidence that IFNalpha induces SOCS expression in human T cells. Moreover, we show that IFNalpha and IL-2 induce distinct patterns of expression kinetics, suggesting that dynamic changes in cytokine sensitivity might be mediated via induction of SOCS expression with different kinetics in T cells.
M3 - Journal article
C2 - 11340296
SN - 0254-9670
VL - 18
SP - 80
EP - 85
JO - Experimental and Clinical Immunogenetics
JF - Experimental and Clinical Immunogenetics
IS - 2
ER -