Insulin tolerance test under anaesthesia to measure tissue-specific insulin-stimulated glucose disposal

Daniel J Fazakerley; Andreas Mæchel Fritzen; Marin E Nelson; Ida H Thorius; Kristen C Cooke; Sean J Humphrey; Gregory J Cooney; David E James

doi:10.21769/BioProtoc.3146

Insulin tolerance test under anaesthesia to measure tissue-specific insulin-stimulated glucose disposal

Daniel J Fazakerley, Andreas Mæchel Fritzen, Marin E Nelson, Ida H Thorius, Kristen C Cooke, Sean J Humphrey, Gregory J Cooney, David E James

The August Krogh Section for Molecular Physiology

50 Downloads (Pure)

Abstract

Insulin resistance is a pathophysiological state defined by impaired responses to insulin and is a risk factor for several metabolic diseases, most notably type 2 diabetes. Insulin resistance occurs in insulin target tissues including liver, adipose and skeletal muscle. Methods such as insulin tolerance tests and hyperinsulinaemic-euglycaemic clamps permit assessment of insulin responses in specific tissues and allow the study of the progression and causes of insulin resistance. Here we detail a protocol for assessing insulin action in adipose and muscle tissues in anesthetized mice administered with insulin intravenously.

Original language	English
Article number	e3146
Journal	Bio-protocol
Volume	9
Issue number	2
Number of pages	13
ISSN	2331-8325
DOIs	https://doi.org/10.21769/BioProtoc.3146
Publication status	Published - 20 Jan 2019

Keywords

Faculty of Science
Insulin
Adipose tissue
Muscle
Glucose transport
Glucose uptake
Insulin tolerance test

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.21769/BioProtoc.3146Licence: CC BY

Fazakerley et al_Bio-protocol_2019_Vol 9(2)_e3146Final published version, 1.26 MBLicence: CC BY

Cite this

@article{2317c616d38743d091861150dfca45a3,

title = "Insulin tolerance test under anaesthesia to measure tissue-specific insulin-stimulated glucose disposal",

abstract = "Insulin resistance is a pathophysiological state defined by impaired responses to insulin and is a risk factor for several metabolic diseases, most notably type 2 diabetes. Insulin resistance occurs in insulin target tissues including liver, adipose and skeletal muscle. Methods such as insulin tolerance tests and hyperinsulinaemic-euglycaemic clamps permit assessment of insulin responses in specific tissues and allow the study of the progression and causes of insulin resistance. Here we detail a protocol for assessing insulin action in adipose and muscle tissues in anesthetized mice administered with insulin intravenously.",

keywords = "Faculty of Science, Insulin, Adipose tissue, Muscle, Glucose transport, Glucose uptake, Insulin tolerance test",

author = "Fazakerley, {Daniel J} and Fritzen, {Andreas M{\ae}chel} and Nelson, {Marin E} and Thorius, {Ida H} and Cooke, {Kristen C} and Humphrey, {Sean J} and Cooney, {Gregory J} and James, {David E}",

note = "CURIS 2019 NEXS 050",

year = "2019",

month = jan,

day = "20",

doi = "10.21769/BioProtoc.3146",

language = "English",

volume = "9",

journal = "Bio-protocol",

issn = "2331-8325",

publisher = "bio-protocol",

number = "2",

}

TY - JOUR

T1 - Insulin tolerance test under anaesthesia to measure tissue-specific insulin-stimulated glucose disposal

AU - Fazakerley, Daniel J

AU - Fritzen, Andreas Mæchel

AU - Nelson, Marin E

AU - Thorius, Ida H

AU - Cooke, Kristen C

AU - Humphrey, Sean J

AU - Cooney, Gregory J

AU - James, David E

N1 - CURIS 2019 NEXS 050

PY - 2019/1/20

Y1 - 2019/1/20

N2 - Insulin resistance is a pathophysiological state defined by impaired responses to insulin and is a risk factor for several metabolic diseases, most notably type 2 diabetes. Insulin resistance occurs in insulin target tissues including liver, adipose and skeletal muscle. Methods such as insulin tolerance tests and hyperinsulinaemic-euglycaemic clamps permit assessment of insulin responses in specific tissues and allow the study of the progression and causes of insulin resistance. Here we detail a protocol for assessing insulin action in adipose and muscle tissues in anesthetized mice administered with insulin intravenously.

AB - Insulin resistance is a pathophysiological state defined by impaired responses to insulin and is a risk factor for several metabolic diseases, most notably type 2 diabetes. Insulin resistance occurs in insulin target tissues including liver, adipose and skeletal muscle. Methods such as insulin tolerance tests and hyperinsulinaemic-euglycaemic clamps permit assessment of insulin responses in specific tissues and allow the study of the progression and causes of insulin resistance. Here we detail a protocol for assessing insulin action in adipose and muscle tissues in anesthetized mice administered with insulin intravenously.

KW - Faculty of Science

KW - Insulin

KW - Adipose tissue

KW - Muscle

KW - Glucose transport

KW - Glucose uptake

KW - Insulin tolerance test

U2 - 10.21769/BioProtoc.3146

DO - 10.21769/BioProtoc.3146

M3 - Journal article

SN - 2331-8325

VL - 9

JO - Bio-protocol

JF - Bio-protocol

IS - 2

M1 - e3146

ER -

Insulin tolerance test under anaesthesia to measure tissue-specific insulin-stimulated glucose disposal

Abstract

Keywords

UN SDGs

Access to Document

Fingerprint

Cite this