Insulin tolerance test under anaesthesia to measure tissue-specific insulin-stimulated glucose disposal

Daniel J Fazakerley; Andreas Mæchel Fritzen; Marin E Nelson; Ida H Thorius; Kristen C Cooke; Sean J Humphrey; Gregory J Cooney; David E James

doi:10.21769/BioProtoc.3146

Insulin tolerance test under anaesthesia to measure tissue-specific insulin-stimulated glucose disposal

Daniel J Fazakerley, Andreas Mæchel Fritzen, Marin E Nelson, Ida H Thorius, Kristen C Cooke, Sean J Humphrey, Gregory J Cooney, David E James

August Krogh Sektionen for Molekylær Fysiologi

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Abstract

Insulin resistance is a pathophysiological state defined by impaired responses to insulin and is a risk factor for several metabolic diseases, most notably type 2 diabetes. Insulin resistance occurs in insulin target tissues including liver, adipose and skeletal muscle. Methods such as insulin tolerance tests and hyperinsulinaemic-euglycaemic clamps permit assessment of insulin responses in specific tissues and allow the study of the progression and causes of insulin resistance. Here we detail a protocol for assessing insulin action in adipose and muscle tissues in anesthetized mice administered with insulin intravenously.

Originalsprog	Engelsk
Artikelnummer	e3146
Tidsskrift	Bio-protocol
Vol/bind	9
Udgave nummer	2
Antal sider	13
ISSN	2331-8325
DOI	https://doi.org/10.21769/BioProtoc.3146
Status	Udgivet - 20 jan. 2019

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Det Natur- og Biovidenskabelige Fakultet

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10.21769/BioProtoc.3146Licens: CC BY

Fazakerley et al_Bio-protocol_2019_Vol 9(2)_e3146Forlagets udgivne version, 1,26 MBLicens: CC BY

Citationsformater

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abstract = "Insulin resistance is a pathophysiological state defined by impaired responses to insulin and is a risk factor for several metabolic diseases, most notably type 2 diabetes. Insulin resistance occurs in insulin target tissues including liver, adipose and skeletal muscle. Methods such as insulin tolerance tests and hyperinsulinaemic-euglycaemic clamps permit assessment of insulin responses in specific tissues and allow the study of the progression and causes of insulin resistance. Here we detail a protocol for assessing insulin action in adipose and muscle tissues in anesthetized mice administered with insulin intravenously.",

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AU - Fazakerley, Daniel J

AU - Fritzen, Andreas Mæchel

AU - Nelson, Marin E

AU - Thorius, Ida H

AU - Cooke, Kristen C

AU - Humphrey, Sean J

AU - Cooney, Gregory J

AU - James, David E

N1 - CURIS 2019 NEXS 050

PY - 2019/1/20

Y1 - 2019/1/20

N2 - Insulin resistance is a pathophysiological state defined by impaired responses to insulin and is a risk factor for several metabolic diseases, most notably type 2 diabetes. Insulin resistance occurs in insulin target tissues including liver, adipose and skeletal muscle. Methods such as insulin tolerance tests and hyperinsulinaemic-euglycaemic clamps permit assessment of insulin responses in specific tissues and allow the study of the progression and causes of insulin resistance. Here we detail a protocol for assessing insulin action in adipose and muscle tissues in anesthetized mice administered with insulin intravenously.

AB - Insulin resistance is a pathophysiological state defined by impaired responses to insulin and is a risk factor for several metabolic diseases, most notably type 2 diabetes. Insulin resistance occurs in insulin target tissues including liver, adipose and skeletal muscle. Methods such as insulin tolerance tests and hyperinsulinaemic-euglycaemic clamps permit assessment of insulin responses in specific tissues and allow the study of the progression and causes of insulin resistance. Here we detail a protocol for assessing insulin action in adipose and muscle tissues in anesthetized mice administered with insulin intravenously.

KW - Faculty of Science

KW - Insulin

KW - Adipose tissue

KW - Muscle

KW - Glucose transport

KW - Glucose uptake

KW - Insulin tolerance test

U2 - 10.21769/BioProtoc.3146

DO - 10.21769/BioProtoc.3146

M3 - Journal article

SN - 2331-8325

VL - 9

JO - Bio-protocol

JF - Bio-protocol

IS - 2

M1 - e3146

ER -

Insulin tolerance test under anaesthesia to measure tissue-specific insulin-stimulated glucose disposal

Abstract

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