Abstract
We examined the simultaneous effects of a 24-h low-grade Intralipid infusion on peripheral glucose disposal, intracellular glucose partitioning and insulin secretion rates in twenty young men, by 2-step hyperinsulinemic euglycemic clamp [low insulin clamp (LI), 10 mU/m(2) x min; high insulin clamp (HI), 40 mU/m(2) x min], 3-(3)H-glucose, indirect calorimetry, and iv glucose tolerance test. Free fatty acid concentrations were similar during basal steady state but 3.7- to 13-fold higher during clamps. P-glucagon increased and the insulin/glucagon ratio decreased at both LI and HI during Intralipid infusion. At LI, glucose oxidation decreased by 10%, whereas glucose disposal, glycolytic flux, glucose storage, and glucose production were not significantly altered. At HI, glucose disposal, and glucose oxidation decreased by 12% and 24%, respectively, during Intralipid infusion. Glycolytic flux, glucose storage, and glucose production were unchanged. Insulin secretion rates increased in response to Intralipid infusion, but disposition indices (DI = insulin action.insulin secretion) were unchanged. In conclusion, a 24-h low-grade Intralipid infusion caused insulin resistance in the oxidative (but not in the nonoxidative) glucose metabolism in young healthy men. Moreover, insulin hypersecretion perfectly countered the free-fatty acid-induced insulin resistance. Future studies are needed to determine the role of a prolonged moderate lipid load in subjects at increased risk of developing diabetes.
| Original language | English |
|---|---|
| Journal | The Journal of clinical endocrinology and metabolism |
| Volume | 88 |
| Issue number | 6 |
| Pages (from-to) | 2775-83 |
| Number of pages | 9 |
| ISSN | 0021-972X |
| DOIs | |
| Publication status | Published - Jun 2003 |
Keywords
- Adult
- Area Under Curve
- Blood Glucose
- C-Peptide
- Fat Emulsions, Intravenous
- Fatty Acids, Nonesterified
- Glucagon
- Glucose
- Glucose Tolerance Test
- Humans
- Insulin
- Insulin Resistance
- Male
- Oxidation-Reduction
