Inhibition of nitric oxide synthesis by systemic N(G)-monomethyl-L-arginine administration in humans: effects on interstitial adenosine, prostacyclin and potassium concentrations in resting and contracting skeletal muscle

U Frandsen; J Bangsbo; Henning Langberg; B Saltin; Y Hellsten

doi:25743

Inhibition of nitric oxide synthesis by systemic N(G)-monomethyl-L-arginine administration in humans: effects on interstitial adenosine, prostacyclin and potassium concentrations in resting and contracting skeletal muscle

U Frandsen, J Bangsbo, Henning Langberg, B Saltin, Y Hellsten

44 Citations (Scopus)

Abstract

We examined whether the formation or the release of the vasodilators adenosine, prostacyclin (PGI(2)) and potassium (K(+)) increase in skeletal muscle interstitium in response to nitric oxide synthase (NOS) inhibition. Five subjects performed one-legged knee extensor exercise at 30 W without (controls) and with prior N(G)-nitro-L-arginine methyl ester (L-NAME) infusion (4 mg/kg, intravenously). Samples from the interstitial fluid were obtained at rest, during exercise and after exercise with the microdialysis technique. Interstitial adenosine in controls increased (p0.05) to controls. The 6-keto-prostaglandin F1alpha concentration in controls was 1.17+/-0.20 ng/ml at rest and increased (p0.05) in L-NAME. The interstitial K(+) concentration in controls increased (p

Original language	English
Journal	Journal of Vascular Research
Volume	37
Issue number	4
Pages (from-to)	297-302
Number of pages	6
ISSN	1018-1172
DOIs	https://doi.org/25743
Publication status	Published - 2000

Keywords

6-Ketoprostaglandin F1 alpha
Adenosine
Adult
Blood Pressure
Enzyme Inhibitors
Epoprostenol
Extracellular Space
Humans
Leg
Male
Muscle Contraction
Muscle, Skeletal
Nitric Oxide
Osmolar Concentration
Potassium
Regional Blood Flow
Rest
omega-N-Methylarginine

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Inhibition of nitric oxide synthesis by systemic N(G)-monomethyl-L-arginine administration in humans : effects on interstitial adenosine, prostacyclin and potassium concentrations in resting and contracting skeletal muscle. / Frandsen, U; Bangsbo, J ; Langberg, Henning et al.

In: Journal of Vascular Research, Vol. 37, No. 4, 2000, p. 297-302.

Research output: Contribution to journal › Journal article › Research › peer-review

Frandsen, U, Bangsbo, J , Langberg, H, Saltin, B & Hellsten, Y 2000, 'Inhibition of nitric oxide synthesis by systemic N(G)-monomethyl-L-arginine administration in humans: effects on interstitial adenosine, prostacyclin and potassium concentrations in resting and contracting skeletal muscle', Journal of Vascular Research, vol. 37, no. 4, pp. 297-302. https://doi.org/25743

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abstract = "We examined whether the formation or the release of the vasodilators adenosine, prostacyclin (PGI(2)) and potassium (K(+)) increase in skeletal muscle interstitium in response to nitric oxide synthase (NOS) inhibition. Five subjects performed one-legged knee extensor exercise at 30 W without (controls) and with prior N(G)-nitro-L-arginine methyl ester (L-NAME) infusion (4 mg/kg, intravenously). Samples from the interstitial fluid were obtained at rest, during exercise and after exercise with the microdialysis technique. Interstitial adenosine in controls increased (p0.05) to controls. The 6-keto-prostaglandin F1alpha concentration in controls was 1.17+/-0.20 ng/ml at rest and increased (p0.05) in L-NAME. The interstitial K(+) concentration in controls increased (p",

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AU - Saltin, B

AU - Hellsten, Y

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N2 - We examined whether the formation or the release of the vasodilators adenosine, prostacyclin (PGI(2)) and potassium (K(+)) increase in skeletal muscle interstitium in response to nitric oxide synthase (NOS) inhibition. Five subjects performed one-legged knee extensor exercise at 30 W without (controls) and with prior N(G)-nitro-L-arginine methyl ester (L-NAME) infusion (4 mg/kg, intravenously). Samples from the interstitial fluid were obtained at rest, during exercise and after exercise with the microdialysis technique. Interstitial adenosine in controls increased (p0.05) to controls. The 6-keto-prostaglandin F1alpha concentration in controls was 1.17+/-0.20 ng/ml at rest and increased (p0.05) in L-NAME. The interstitial K(+) concentration in controls increased (p

AB - We examined whether the formation or the release of the vasodilators adenosine, prostacyclin (PGI(2)) and potassium (K(+)) increase in skeletal muscle interstitium in response to nitric oxide synthase (NOS) inhibition. Five subjects performed one-legged knee extensor exercise at 30 W without (controls) and with prior N(G)-nitro-L-arginine methyl ester (L-NAME) infusion (4 mg/kg, intravenously). Samples from the interstitial fluid were obtained at rest, during exercise and after exercise with the microdialysis technique. Interstitial adenosine in controls increased (p0.05) to controls. The 6-keto-prostaglandin F1alpha concentration in controls was 1.17+/-0.20 ng/ml at rest and increased (p0.05) in L-NAME. The interstitial K(+) concentration in controls increased (p

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KW - Blood Pressure

KW - Enzyme Inhibitors

KW - Epoprostenol

KW - Extracellular Space

KW - Humans

KW - Leg

KW - Male

KW - Muscle Contraction

KW - Muscle, Skeletal

KW - Nitric Oxide

KW - Osmolar Concentration

KW - Potassium

KW - Regional Blood Flow

KW - Rest

KW - omega-N-Methylarginine

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