Abstract
A 5 mg/mL solution of water, methanol and acetone extracts of seaweeds were used for α-glucosidase inhibition assay hyphenated with high performance liquid chromatography-mass spectrometry (HPLC-HRMS). The results showed acetone extracts of Undaria pinnatifida has the strongest inhibitory effect against α-glucosidase activity with IC50 0.08 ± 0.002 mg/mL. The active compound found in Undaria pinnatifida was identified as fucoxanthin. Analytical standard sample of fucoxanthin significantly inhibited α-glucosidase with IC50 value 0.047 ± 0.001 mg/mL. An inhibition kinetics study indicates that fucoxanthin is showing mixed-type inhibition. These results suggest that Undaria pinnatifida has a potential to inhibit α-glucosidase and may be used as a bioactive food ingredient for glycaemic control.
| Original language | English |
|---|---|
| Journal | Food Chemistry |
| Volume | 270 |
| Pages (from-to) | 481-486 |
| Number of pages | 6 |
| ISSN | 0308-8146 |
| DOIs | |
| Publication status | Published - 1 Jan 2019 |
Keywords
- Faculty of Science
- Seaweed
- Glycaemic control
- Hyperglycaemia
- α-Glucosidase
- Fucoxanthin