Individual predisposition to Staphylococcus aureus colonization in pigs based on quantification, carriage dynamics and serological profiles.

Carmen Espinosa Gongora; Jan Dahl; Anders Elvstrøm; Willem J. van Wamel; Luca Guardabassi

doi:10.1128/aem.03392-14

Individual predisposition to Staphylococcus aureus colonization in pigs based on quantification, carriage dynamics and serological profiles.

Carmen Espinosa Gongora, Jan Dahl, Anders Elvstrøm, Willem J. van Wamel, Luca Guardabassi

10 Citations (Scopus)

Abstract

Previous research on Staphylococcus aureus in pigs focused on livestock-associated methicillin-resistant S. aureus (MRSA) and had a qualitative cross-sectional design. This study aimed to elucidate the frequency, load, and stability of S. aureus nasal carriage in pigs over time and investigated possible associations between carriage and immune response. Nasal swabs were collected three times weekly from 480 tagged adult pigs in 20 Danish production farms. S. aureus and MRSA were quantified on selective media by the most-probable-number method. The levels of IgG against 10 S. aureus antigens in serum were quantified in selected pigs by a Luminex assay. All the farms were positive for S. aureus and 15 for MRSA, leading to overall prevalences of persistent and intermittent carriers and noncarriers of 24, 52, and 23%, respectively. Carriage frequency and nasal loads were significantly higher on MRSA-positive farms. Logistic-regression modeling revealed the presence of individual pigs characterized by high nasal loads (≥ 10,000 CFU per swab) and stable carriage regardless of farm-and pen-associated factors. On the other hand, the humoral response was strongly influenced by these environmental factors. The existence of a minority of shedders contributing to maintenance of S. aureus within farms opens up new perspectives on the control of MRSA in pig farming.

Original language	English
Journal	Applied and Environmental Microbiology
Volume	81
Issue number	4
Pages (from-to)	1251-1256
Number of pages	6
ISSN	0099-2240
DOIs	https://doi.org/10.1128/aem.03392-14
Publication status	Published - Feb 2015

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Individual predisposition to Staphylococcus aureus colonization in pigs based on quantification, carriage dynamics and serological profiles. / Gongora, Carmen Espinosa; Dahl, Jan; Elvstrøm, Anders et al.

In: Applied and Environmental Microbiology, Vol. 81, No. 4, 02.2015, p. 1251-1256.

Research output: Contribution to journal › Journal article › Research › peer-review

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title = "Individual predisposition to Staphylococcus aureus colonization in pigs based on quantification, carriage dynamics and serological profiles.",

abstract = "Previous research on Staphylococcus aureus in pigs focused on livestock-associated methicillin-resistant S. aureus (MRSA) and had a qualitative cross-sectional design. This study aimed to elucidate the frequency, load, and stability of S. aureus nasal carriage in pigs over time and investigated possible associations between carriage and immune response. Nasal swabs were collected three times weekly from 480 tagged adult pigs in 20 Danish production farms. S. aureus and MRSA were quantified on selective media by the most-probable-number method. The levels of IgG against 10 S. aureus antigens in serum were quantified in selected pigs by a Luminex assay. All the farms were positive for S. aureus and 15 for MRSA, leading to overall prevalences of persistent and intermittent carriers and noncarriers of 24, 52, and 23%, respectively. Carriage frequency and nasal loads were significantly higher on MRSA-positive farms. Logistic-regression modeling revealed the presence of individual pigs characterized by high nasal loads (≥ 10,000 CFU per swab) and stable carriage regardless of farm-and pen-associated factors. On the other hand, the humoral response was strongly influenced by these environmental factors. The existence of a minority of shedders contributing to maintenance of S. aureus within farms opens up new perspectives on the control of MRSA in pig farming.",

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AU - Elvstrøm, Anders

AU - van Wamel, Willem J.

AU - Guardabassi, Luca

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N2 - Previous research on Staphylococcus aureus in pigs focused on livestock-associated methicillin-resistant S. aureus (MRSA) and had a qualitative cross-sectional design. This study aimed to elucidate the frequency, load, and stability of S. aureus nasal carriage in pigs over time and investigated possible associations between carriage and immune response. Nasal swabs were collected three times weekly from 480 tagged adult pigs in 20 Danish production farms. S. aureus and MRSA were quantified on selective media by the most-probable-number method. The levels of IgG against 10 S. aureus antigens in serum were quantified in selected pigs by a Luminex assay. All the farms were positive for S. aureus and 15 for MRSA, leading to overall prevalences of persistent and intermittent carriers and noncarriers of 24, 52, and 23%, respectively. Carriage frequency and nasal loads were significantly higher on MRSA-positive farms. Logistic-regression modeling revealed the presence of individual pigs characterized by high nasal loads (≥ 10,000 CFU per swab) and stable carriage regardless of farm-and pen-associated factors. On the other hand, the humoral response was strongly influenced by these environmental factors. The existence of a minority of shedders contributing to maintenance of S. aureus within farms opens up new perspectives on the control of MRSA in pig farming.

AB - Previous research on Staphylococcus aureus in pigs focused on livestock-associated methicillin-resistant S. aureus (MRSA) and had a qualitative cross-sectional design. This study aimed to elucidate the frequency, load, and stability of S. aureus nasal carriage in pigs over time and investigated possible associations between carriage and immune response. Nasal swabs were collected three times weekly from 480 tagged adult pigs in 20 Danish production farms. S. aureus and MRSA were quantified on selective media by the most-probable-number method. The levels of IgG against 10 S. aureus antigens in serum were quantified in selected pigs by a Luminex assay. All the farms were positive for S. aureus and 15 for MRSA, leading to overall prevalences of persistent and intermittent carriers and noncarriers of 24, 52, and 23%, respectively. Carriage frequency and nasal loads were significantly higher on MRSA-positive farms. Logistic-regression modeling revealed the presence of individual pigs characterized by high nasal loads (≥ 10,000 CFU per swab) and stable carriage regardless of farm-and pen-associated factors. On the other hand, the humoral response was strongly influenced by these environmental factors. The existence of a minority of shedders contributing to maintenance of S. aureus within farms opens up new perspectives on the control of MRSA in pig farming.

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DO - 10.1128/aem.03392-14

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JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

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Individual predisposition to Staphylococcus aureus colonization in pigs based on quantification, carriage dynamics and serological profiles.

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