Independent control of replication initiation of the two Vibrio cholerae chromosomes by DnaA and RctB

Stéphane Duigou; Kristine G. Knudsen; Ole Skovgaard; Elizabeth S Egan; Anders Løbner-Olesen; Matthew K Waldor

doi:10.1128/JB.00565-06

Independent control of replication initiation of the two Vibrio cholerae chromosomes by DnaA and RctB

Stéphane Duigou, Kristine G. Knudsen, Ole Skovgaard, Elizabeth S Egan, Anders Løbner-Olesen, Matthew K Waldor

60 Citations (Scopus)

Abstract

Although the two Vibrio cholerae chromosomes initiate replication in a coordinated fashion, we show here that each chromosome appears to have a specific replication initiator. DnaA overproduction promoted overinitiation of chromosome I and not chromosome II. In contrast, overproduction of RctB, a protein that binds to the origin of replication of chromosome II, promoted overinitiation of chromosome II and not chromosome I.

Original language	English
Journal	Journal of Bacteriology
Volume	188
Issue number	17
Pages (from-to)	6419-6424
Number of pages	6
ISSN	0021-9193
DOIs	https://doi.org/10.1128/JB.00565-06
Publication status	Published - 2006
Externally published	Yes

Keywords

Bacterial Proteins/physiology
Chromosomes, Bacterial/genetics
DNA Replication
DNA-Binding Proteins/physiology
Vibrio cholerae/genetics

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10.1128/JB.00565-06

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title = "Independent control of replication initiation of the two Vibrio cholerae chromosomes by DnaA and RctB",

abstract = "Although the two Vibrio cholerae chromosomes initiate replication in a coordinated fashion, we show here that each chromosome appears to have a specific replication initiator. DnaA overproduction promoted overinitiation of chromosome I and not chromosome II. In contrast, overproduction of RctB, a protein that binds to the origin of replication of chromosome II, promoted overinitiation of chromosome II and not chromosome I.",

keywords = "Bacterial Proteins/physiology, Chromosomes, Bacterial/genetics, DNA Replication, DNA-Binding Proteins/physiology, Vibrio cholerae/genetics",

author = "St{\'e}phane Duigou and Knudsen, {Kristine G.} and Ole Skovgaard and Egan, {Elizabeth S} and Anders L{\o}bner-Olesen and Waldor, {Matthew K}",

year = "2006",

doi = "10.1128/JB.00565-06",

language = "English",

volume = "188",

pages = "6419--6424",

journal = "Journal of Bacteriology",

issn = "0021-9193",

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TY - JOUR

T1 - Independent control of replication initiation of the two Vibrio cholerae chromosomes by DnaA and RctB

AU - Duigou, Stéphane

AU - Knudsen, Kristine G.

AU - Skovgaard, Ole

AU - Egan, Elizabeth S

AU - Løbner-Olesen, Anders

AU - Waldor, Matthew K

PY - 2006

Y1 - 2006

N2 - Although the two Vibrio cholerae chromosomes initiate replication in a coordinated fashion, we show here that each chromosome appears to have a specific replication initiator. DnaA overproduction promoted overinitiation of chromosome I and not chromosome II. In contrast, overproduction of RctB, a protein that binds to the origin of replication of chromosome II, promoted overinitiation of chromosome II and not chromosome I.

AB - Although the two Vibrio cholerae chromosomes initiate replication in a coordinated fashion, we show here that each chromosome appears to have a specific replication initiator. DnaA overproduction promoted overinitiation of chromosome I and not chromosome II. In contrast, overproduction of RctB, a protein that binds to the origin of replication of chromosome II, promoted overinitiation of chromosome II and not chromosome I.

KW - Bacterial Proteins/physiology

KW - Chromosomes, Bacterial/genetics

KW - DNA Replication

KW - DNA-Binding Proteins/physiology

KW - Vibrio cholerae/genetics

U2 - 10.1128/JB.00565-06

DO - 10.1128/JB.00565-06

M3 - Journal article

C2 - 16923911

SN - 0021-9193

VL - 188

SP - 6419

EP - 6424

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 17

ER -

Independent control of replication initiation of the two Vibrio cholerae chromosomes by DnaA and RctB

Abstract

Keywords

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