Increased subventricular zone-derived cortical neurogenesis after ischemic lesion

TY - JOUR

T1 - Increased subventricular zone-derived cortical neurogenesis after ischemic lesion

AU - Kreuzberg, Maria

AU - Kanov, Evgeny

AU - Timofeev, Oleg

AU - Schwaninger, Markus

AU - Monyer, Hannah

AU - Khodosevich, Konstantin

N1 - Copyright © 2010 Elsevier Inc. All rights reserved.

PY - 2010/11

Y1 - 2010/11

N2 - In adult rodents stroke enhances neurogenesis resulting in the addition of neurons to forebrain regions such as striatum or cortex where postnatal neurogenesis under normal conditions plays a negligible role. In the cortex, new neurons are generated either from local cortical precursors that are activated by stroke or from precursors residing in the subventricular zone (SVZ) of lateral ventricles that under normal conditions supply neuroblasts by and large only for the olfactory bulb. In this study we used 5HT3A-EGFP transgenic mice in which all neuroblasts originating in the SVZ are EGFP-labeled. We induced stroke in these mice and by combination of EGFP detection with BrdU injections we labeled all post-stroke-generated SVZ-derived neuroblasts. We showed an increase in SVZ-derived neuroblasts 14 and 35 days after stroke in the ipsilateral hemisphere. Post-stroke-generated SVZ-derived neuroblasts migrated to the cortex and survived for at least 35 days representing 2% of BrdU-positive cells in peri-infarct area where they differentiate into mature neurons. Thus, stroke enhances SVZ neurogenesis and attracts newborn neurons to the injury zone.

AB - In adult rodents stroke enhances neurogenesis resulting in the addition of neurons to forebrain regions such as striatum or cortex where postnatal neurogenesis under normal conditions plays a negligible role. In the cortex, new neurons are generated either from local cortical precursors that are activated by stroke or from precursors residing in the subventricular zone (SVZ) of lateral ventricles that under normal conditions supply neuroblasts by and large only for the olfactory bulb. In this study we used 5HT3A-EGFP transgenic mice in which all neuroblasts originating in the SVZ are EGFP-labeled. We induced stroke in these mice and by combination of EGFP detection with BrdU injections we labeled all post-stroke-generated SVZ-derived neuroblasts. We showed an increase in SVZ-derived neuroblasts 14 and 35 days after stroke in the ipsilateral hemisphere. Post-stroke-generated SVZ-derived neuroblasts migrated to the cortex and survived for at least 35 days representing 2% of BrdU-positive cells in peri-infarct area where they differentiate into mature neurons. Thus, stroke enhances SVZ neurogenesis and attracts newborn neurons to the injury zone.

KW - Animals

KW - Antimetabolites

KW - Apoptosis/physiology

KW - Brain Ischemia/pathology

KW - Bromodeoxyuridine

KW - Calbindin 2

KW - Caspase 3/biosynthesis

KW - Cerebral Cortex/cytology

KW - Cerebral Ventricles/cytology

KW - Green Fluorescent Proteins/genetics

KW - Immunohistochemistry

KW - Infarction, Middle Cerebral Artery/pathology

KW - Interneurons/physiology

KW - Mice

KW - Mice, Transgenic

KW - Nerve Tissue Proteins/biosynthesis

KW - Neurogenesis/physiology

KW - Neurons/physiology

KW - Nuclear Proteins/biosynthesis

KW - Prefrontal Cortex/pathology

KW - Receptors, Serotonin, 5-HT3/biosynthesis

KW - S100 Calcium Binding Protein G/metabolism

KW - gamma-Aminobutyric Acid/physiology

U2 - 10.1016/j.expneurol.2010.08.006

DO - 10.1016/j.expneurol.2010.08.006

M3 - Journal article

C2 - 20713052

SN - 0014-4886

VL - 226

SP - 90

EP - 99

JO - Experimental Neurology

JF - Experimental Neurology

IS - 1

ER -