TY - JOUR
T1 - Increased subventricular zone-derived cortical neurogenesis after ischemic lesion
AU - Kreuzberg, Maria
AU - Kanov, Evgeny
AU - Timofeev, Oleg
AU - Schwaninger, Markus
AU - Monyer, Hannah
AU - Khodosevich, Konstantin
N1 - Copyright © 2010 Elsevier Inc. All rights reserved.
PY - 2010/11
Y1 - 2010/11
N2 - In adult rodents stroke enhances neurogenesis resulting in the addition of neurons to forebrain regions such as striatum or cortex where postnatal neurogenesis under normal conditions plays a negligible role. In the cortex, new neurons are generated either from local cortical precursors that are activated by stroke or from precursors residing in the subventricular zone (SVZ) of lateral ventricles that under normal conditions supply neuroblasts by and large only for the olfactory bulb. In this study we used 5HT3A-EGFP transgenic mice in which all neuroblasts originating in the SVZ are EGFP-labeled. We induced stroke in these mice and by combination of EGFP detection with BrdU injections we labeled all post-stroke-generated SVZ-derived neuroblasts. We showed an increase in SVZ-derived neuroblasts 14 and 35 days after stroke in the ipsilateral hemisphere. Post-stroke-generated SVZ-derived neuroblasts migrated to the cortex and survived for at least 35 days representing 2% of BrdU-positive cells in peri-infarct area where they differentiate into mature neurons. Thus, stroke enhances SVZ neurogenesis and attracts newborn neurons to the injury zone.
AB - In adult rodents stroke enhances neurogenesis resulting in the addition of neurons to forebrain regions such as striatum or cortex where postnatal neurogenesis under normal conditions plays a negligible role. In the cortex, new neurons are generated either from local cortical precursors that are activated by stroke or from precursors residing in the subventricular zone (SVZ) of lateral ventricles that under normal conditions supply neuroblasts by and large only for the olfactory bulb. In this study we used 5HT3A-EGFP transgenic mice in which all neuroblasts originating in the SVZ are EGFP-labeled. We induced stroke in these mice and by combination of EGFP detection with BrdU injections we labeled all post-stroke-generated SVZ-derived neuroblasts. We showed an increase in SVZ-derived neuroblasts 14 and 35 days after stroke in the ipsilateral hemisphere. Post-stroke-generated SVZ-derived neuroblasts migrated to the cortex and survived for at least 35 days representing 2% of BrdU-positive cells in peri-infarct area where they differentiate into mature neurons. Thus, stroke enhances SVZ neurogenesis and attracts newborn neurons to the injury zone.
KW - Animals
KW - Antimetabolites
KW - Apoptosis/physiology
KW - Brain Ischemia/pathology
KW - Bromodeoxyuridine
KW - Calbindin 2
KW - Caspase 3/biosynthesis
KW - Cerebral Cortex/cytology
KW - Cerebral Ventricles/cytology
KW - Green Fluorescent Proteins/genetics
KW - Immunohistochemistry
KW - Infarction, Middle Cerebral Artery/pathology
KW - Interneurons/physiology
KW - Mice
KW - Mice, Transgenic
KW - Nerve Tissue Proteins/biosynthesis
KW - Neurogenesis/physiology
KW - Neurons/physiology
KW - Nuclear Proteins/biosynthesis
KW - Prefrontal Cortex/pathology
KW - Receptors, Serotonin, 5-HT3/biosynthesis
KW - S100 Calcium Binding Protein G/metabolism
KW - gamma-Aminobutyric Acid/physiology
U2 - 10.1016/j.expneurol.2010.08.006
DO - 10.1016/j.expneurol.2010.08.006
M3 - Journal article
C2 - 20713052
SN - 0014-4886
VL - 226
SP - 90
EP - 99
JO - Experimental Neurology
JF - Experimental Neurology
IS - 1
ER -