Increased number and frequency of group 3 innate lymphoid cells in nonlesional psoriatic skin

TY - JOUR

T1 - Increased number and frequency of group 3 innate lymphoid cells in nonlesional psoriatic skin

AU - Dyring-Andersen, B

AU - Geisler, Carsten

AU - Agerbeck, C

AU - Lauritsen, J P H

AU - Gúdjonsdottir, S D

AU - Skov, L

AU - Bonefeld, C M

N1 - © 2013 British Association of Dermatologists.

PY - 2014/3

Y1 - 2014/3

N2 - Background Psoriasis is a common immune-mediated inflammatory disease that affects the skin and joints. The interleukin (IL)-23/IL-17A axis and IL-22 play key roles in the pathogenesis of psoriasis. IL-23-responsive innate lymphoid cells (ILCs) with a high capacity to produce IL-17 and/or IL-22 have recently been identified and associated with inflammatory bowel diseases. The occurrence and role of ILCs in human skin are poorly understood. Objectives To describe the prevalence of the different ILC subpopulations in skin from healthy controls and patients with psoriasis or allergy to nickel. Methods Skin biopsies were taken from healthy skin, nonlesional and lesional psoriatic skin, and nickel- and petrolatum-exposed skin from patients with contact allergy to nickel, and lymphocytes were isolated. The cells were stained and characterized by flow cytometry. Cytokine and ligand mRNA expression were measured by quantitative polymerase chain reaction. Results We found that members of the three groups of ILCs were present in human skin. Remarkably, the number and frequency of RORγt+CD56+ ILC3s, which are known to produce IL-22, were elevated in both nonlesional and lesional skin from patients with psoriasis compared with healthy skin and skin from patients with contact allergy to nickel. Furthermore, skin ILCs expressed high levels of the natural killer receptor NKG2D. NKG2D binds to stress-induced ligands, including major histocompatibility complex class I-related chain A, which we found to be strongly upregulated in lesional skin from patients with psoriasis. Conclusion These results show that ILCs are present in human skin and indicate that RORγt+CD56+ ILC3 may be involved in the pathogenesis of psoriasis. What's already known about this topic? The interleukin (IL)-23/Th17 axis is important for the pathogenesis of psoriasis. Innate lymphoid cells producing IL-17 and/or IL-22 are known to be involved in the pathogenesis of psoriasis. What does this study add? An increased frequency of group 3 innate lymphoid cells is found in noninvolved psoriatic skin compared with healthy skin, which may indicate that these cells contribute to psoriasis development.

AB - Background Psoriasis is a common immune-mediated inflammatory disease that affects the skin and joints. The interleukin (IL)-23/IL-17A axis and IL-22 play key roles in the pathogenesis of psoriasis. IL-23-responsive innate lymphoid cells (ILCs) with a high capacity to produce IL-17 and/or IL-22 have recently been identified and associated with inflammatory bowel diseases. The occurrence and role of ILCs in human skin are poorly understood. Objectives To describe the prevalence of the different ILC subpopulations in skin from healthy controls and patients with psoriasis or allergy to nickel. Methods Skin biopsies were taken from healthy skin, nonlesional and lesional psoriatic skin, and nickel- and petrolatum-exposed skin from patients with contact allergy to nickel, and lymphocytes were isolated. The cells were stained and characterized by flow cytometry. Cytokine and ligand mRNA expression were measured by quantitative polymerase chain reaction. Results We found that members of the three groups of ILCs were present in human skin. Remarkably, the number and frequency of RORγt+CD56+ ILC3s, which are known to produce IL-22, were elevated in both nonlesional and lesional skin from patients with psoriasis compared with healthy skin and skin from patients with contact allergy to nickel. Furthermore, skin ILCs expressed high levels of the natural killer receptor NKG2D. NKG2D binds to stress-induced ligands, including major histocompatibility complex class I-related chain A, which we found to be strongly upregulated in lesional skin from patients with psoriasis. Conclusion These results show that ILCs are present in human skin and indicate that RORγt+CD56+ ILC3 may be involved in the pathogenesis of psoriasis. What's already known about this topic? The interleukin (IL)-23/Th17 axis is important for the pathogenesis of psoriasis. Innate lymphoid cells producing IL-17 and/or IL-22 are known to be involved in the pathogenesis of psoriasis. What does this study add? An increased frequency of group 3 innate lymphoid cells is found in noninvolved psoriatic skin compared with healthy skin, which may indicate that these cells contribute to psoriasis development.

KW - Case-Control Studies

KW - Dermatitis, Allergic Contact

KW - Humans

KW - Interleukin-23

KW - Interleukins

KW - Killer Cells, Natural

KW - Lymphocyte Subsets

KW - Lymphocytosis

KW - NK Cell Lectin-Like Receptor Subfamily K

KW - Nickel

KW - Nuclear Receptor Subfamily 1, Group F, Member 3

KW - Psoriasis

KW - RNA, Messenger

U2 - 10.1111/bjd.12658

DO - 10.1111/bjd.12658

M3 - Journal article

C2 - 24125475

SN - 0007-0963

VL - 170

SP - 609

EP - 616

JO - British Journal of Dermatology

JF - British Journal of Dermatology

IS - 3

ER -