Increased expression of endothelin B receptor in static stretch exposed porcine mitral valve leaflets

Lotte Gam Pedersen; J. Zhao; J. Yang; Preben Dybdahl Thomsen; H. Gregersen; J.M. Hasenkam; M. Smerup; H.D. Pedersen; Lisbeth Høier Olsen

doi:10.1016/j.rvsc.2006.07.009

Increased expression of endothelin B receptor in static stretch exposed porcine mitral valve leaflets

Lotte Gam Pedersen, J. Zhao, J. Yang, Preben Dybdahl Thomsen, H. Gregersen, J.M. Hasenkam, M. Smerup, H.D. Pedersen, Lisbeth Høier Olsen

8 Citations (Scopus)

Abstract

The aim of this study was to evaluate the effect of mechanical stretch on the expression of ET-1 and ET_A- and ET_B-receptors in porcine mitral valve leaflets.

Leaflet segments from 10 porcine mitral valves were exposed to a static stretch load of 1.5 N for 3.5 h in buffer at 37^oC together with matching control segments. Subsequently, the mRNA expression of ET-1, ET_A-R and ET_B-R was measured by real-time RT-PCR in the chordal insertion areas.

The analyses showed an increased transcription of ET_B-receptors in stretch-exposed leaflet segments compared to unstretched segments median 2.23 (quartiles 1.37 and 2.70) vs. median 1.56 (quartiles 1.38 and 2.17, P=0.03) whereas the mRNA expression of ET_A-receptors (P=0.90) and ET-1 (P=0.51) remained unchanged.

Stretch increased the expression of ET_B-receptors in porcine mitral valve leaflets. The finding could lead to a better understanding of the pathogenesis of myxomatous mitral valve disease.

Original language	English
Journal	Research in Veterinary Science
Volume	82
Issue number	2
Pages (from-to)	232-238
Number of pages	7
ISSN	0034-5288
DOIs	https://doi.org/10.1016/j.rvsc.2006.07.009
Publication status	Published - 2007

Keywords

Former LIFE faculty
Endothelin
Endothelin receptors
Mitral valve
Real-time PCR
Stress
Stretch

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10.1016/j.rvsc.2006.07.009

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@article{6eb09810a1c111ddb6ae000ea68e967b,

title = "Increased expression of endothelin B receptor in static stretch exposed porcine mitral valve leaflets",

abstract = "The aim of this study was to evaluate the effect of mechanical stretch on the expression of ET-1 and ETA- and ETB-receptors in porcine mitral valve leaflets.Leaflet segments from 10 porcine mitral valves were exposed to a static stretch load of 1.5 N for 3.5 h in buffer at 37oC together with matching control segments. Subsequently, the mRNA expression of ET-1, ETA-R and ETB-R was measured by real-time RT-PCR in the chordal insertion areas.The analyses showed an increased transcription of ETB-receptors in stretch-exposed leaflet segments compared to unstretched segments median 2.23 (quartiles 1.37 and 2.70) vs. median 1.56 (quartiles 1.38 and 2.17, P=0.03) whereas the mRNA expression of ETA-receptors (P=0.90) and ET-1 (P=0.51) remained unchanged.Stretch increased the expression of ETB-receptors in porcine mitral valve leaflets. The finding could lead to a better understanding of the pathogenesis of myxomatous mitral valve disease.",

keywords = "Former LIFE faculty, Endothelin, Endothelin receptors, Mitral valve, Real-time PCR, Stress, Stretch",

author = "Pedersen, {Lotte Gam} and J. Zhao and J. Yang and Thomsen, {Preben Dybdahl} and H. Gregersen and J.M. Hasenkam and M. Smerup and H.D. Pedersen and Olsen, {Lisbeth H{\o}ier}",

year = "2007",

doi = "10.1016/j.rvsc.2006.07.009",

language = "English",

volume = "82",

pages = "232--238",

journal = "Research in Veterinary Science",

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T1 - Increased expression of endothelin B receptor in static stretch exposed porcine mitral valve leaflets

AU - Pedersen, Lotte Gam

AU - Zhao, J.

AU - Yang, J.

AU - Thomsen, Preben Dybdahl

AU - Gregersen, H.

AU - Hasenkam, J.M.

AU - Smerup, M.

AU - Pedersen, H.D.

AU - Olsen, Lisbeth Høier

PY - 2007

Y1 - 2007

N2 - The aim of this study was to evaluate the effect of mechanical stretch on the expression of ET-1 and ETA- and ETB-receptors in porcine mitral valve leaflets.Leaflet segments from 10 porcine mitral valves were exposed to a static stretch load of 1.5 N for 3.5 h in buffer at 37oC together with matching control segments. Subsequently, the mRNA expression of ET-1, ETA-R and ETB-R was measured by real-time RT-PCR in the chordal insertion areas.The analyses showed an increased transcription of ETB-receptors in stretch-exposed leaflet segments compared to unstretched segments median 2.23 (quartiles 1.37 and 2.70) vs. median 1.56 (quartiles 1.38 and 2.17, P=0.03) whereas the mRNA expression of ETA-receptors (P=0.90) and ET-1 (P=0.51) remained unchanged.Stretch increased the expression of ETB-receptors in porcine mitral valve leaflets. The finding could lead to a better understanding of the pathogenesis of myxomatous mitral valve disease.

AB - The aim of this study was to evaluate the effect of mechanical stretch on the expression of ET-1 and ETA- and ETB-receptors in porcine mitral valve leaflets.Leaflet segments from 10 porcine mitral valves were exposed to a static stretch load of 1.5 N for 3.5 h in buffer at 37oC together with matching control segments. Subsequently, the mRNA expression of ET-1, ETA-R and ETB-R was measured by real-time RT-PCR in the chordal insertion areas.The analyses showed an increased transcription of ETB-receptors in stretch-exposed leaflet segments compared to unstretched segments median 2.23 (quartiles 1.37 and 2.70) vs. median 1.56 (quartiles 1.38 and 2.17, P=0.03) whereas the mRNA expression of ETA-receptors (P=0.90) and ET-1 (P=0.51) remained unchanged.Stretch increased the expression of ETB-receptors in porcine mitral valve leaflets. The finding could lead to a better understanding of the pathogenesis of myxomatous mitral valve disease.

KW - Former LIFE faculty

KW - Endothelin

KW - Endothelin receptors

KW - Mitral valve

KW - Real-time PCR

KW - Stress

KW - Stretch

U2 - 10.1016/j.rvsc.2006.07.009

DO - 10.1016/j.rvsc.2006.07.009

M3 - Journal article

C2 - 17011002

SN - 0034-5288

VL - 82

SP - 232

EP - 238

JO - Research in Veterinary Science

JF - Research in Veterinary Science

IS - 2

ER -

Increased expression of endothelin B receptor in static stretch exposed porcine mitral valve leaflets

Abstract

Keywords

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