Increased expression of endothelin B receptor in static stretch exposed porcine mitral valve leaflets

TY - JOUR

T1 - Increased expression of endothelin B receptor in static stretch exposed porcine mitral valve leaflets

AU - Pedersen, Lotte Gam

AU - Zhao, J.

AU - Yang, J.

AU - Thomsen, Preben Dybdahl

AU - Gregersen, H.

AU - Hasenkam, J.M.

AU - Smerup, M.

AU - Pedersen, H.D.

AU - Olsen, Lisbeth Høier

PY - 2007

Y1 - 2007

N2 - The aim of this study was to evaluate the effect of mechanical stretch on the expression of ET-1 and ETA- and ETB-receptors in porcine mitral valve leaflets.Leaflet segments from 10 porcine mitral valves were exposed to a static stretch load of 1.5 N for 3.5 h in buffer at 37oC together with matching control segments. Subsequently, the mRNA expression of ET-1, ETA-R and ETB-R was measured by real-time RT-PCR in the chordal insertion areas.The analyses showed an increased transcription of ETB-receptors in stretch-exposed leaflet segments compared to unstretched segments median 2.23 (quartiles 1.37 and 2.70) vs. median 1.56 (quartiles 1.38 and 2.17, P=0.03) whereas the mRNA expression of ETA-receptors (P=0.90) and ET-1 (P=0.51) remained unchanged.Stretch increased the expression of ETB-receptors in porcine mitral valve leaflets. The finding could lead to a better understanding of the pathogenesis of myxomatous mitral valve disease.

AB - The aim of this study was to evaluate the effect of mechanical stretch on the expression of ET-1 and ETA- and ETB-receptors in porcine mitral valve leaflets.Leaflet segments from 10 porcine mitral valves were exposed to a static stretch load of 1.5 N for 3.5 h in buffer at 37oC together with matching control segments. Subsequently, the mRNA expression of ET-1, ETA-R and ETB-R was measured by real-time RT-PCR in the chordal insertion areas.The analyses showed an increased transcription of ETB-receptors in stretch-exposed leaflet segments compared to unstretched segments median 2.23 (quartiles 1.37 and 2.70) vs. median 1.56 (quartiles 1.38 and 2.17, P=0.03) whereas the mRNA expression of ETA-receptors (P=0.90) and ET-1 (P=0.51) remained unchanged.Stretch increased the expression of ETB-receptors in porcine mitral valve leaflets. The finding could lead to a better understanding of the pathogenesis of myxomatous mitral valve disease.

KW - Former LIFE faculty

KW - Endothelin

KW - Endothelin receptors

KW - Mitral valve

KW - Real-time PCR

KW - Stress

KW - Stretch

U2 - 10.1016/j.rvsc.2006.07.009

DO - 10.1016/j.rvsc.2006.07.009

M3 - Journal article

C2 - 17011002

SN - 0034-5288

VL - 82

SP - 232

EP - 238

JO - Research in Veterinary Science

JF - Research in Veterinary Science

IS - 2

ER -