Abstract
The synthesis of a diaminopurine PNA monomer, N-[N6-(benzyloxycarbonyl)-2,6-diaminopurine-9-yl] acetyl-N-(2-t-butyloxycarbonylaminoethyl)glycine, and the incorporation of this monomer into PNA oligomers are described. Substitution of adenine by diaminopurine in PNA oligomers increased the T m of duplexes formed with complementary DNA, RNA or PNA by 2.5-6.5 degrees C per diaminopurine. Furthermore, discrimination against mismatches facing the diaminopurine in the hybridizing oligomer is improved. Finally, a homopurine decamer PNA containing six diaminopurines is shown to form a (gel shift) stable strand displacement complex with a target in a 246 bp double-stranded DNA fragment.
| Original language | English |
|---|---|
| Journal | Nucleic Acids Research |
| Volume | 25 |
| Issue number | 22 |
| Pages (from-to) | 4639-43 |
| Number of pages | 5 |
| ISSN | 0305-1048 |
| Publication status | Published - 15 Nov 1997 |
Keywords
- 2-Aminopurine/analogs & derivatives
- Adenine
- DNA/metabolism
- Molecular Mimicry
- Nucleic Acids/chemical synthesis
- Nylons/chemical synthesis
- Peptides/chemistry