Abstract
Purpose: The purpose of this study is to investigate exercise-induced cellular proliferation in rat skeletal muscle/tendon with the use of 3′-[F-18]fluoro-3′deoxythymidine (FLT) and to quantitatively study concomitant changes in the proliferation-associated factor, Ki67. Procedures: Wistar rats (n∈=∈13) performed 3 days of treadmill running. Cellular proliferation was investigated 3 days before and 48 h after the running exercise with the use of FLT and positron emission tomography/computed tomography (PET/CT). Results were compared to a sedentary control group (n∈=∈10). Image-derived standardized uptake values were calculated for Achilles tendons and calf muscles and compared to gene expression and immunohistochemical evaluations of Ki67. Results: Treadmill running induced increased uptake of FLT uptake in calf muscles (30%; p∈<∈0.001) and in Achilles tendon (21%, p∈<∈0.001). The image-derived results were supported by a correlation in calf muscle to Ki67 (protein and mRNA level), while this coherence was not found in tendon. Conclusion: FLT-PET seems to be a promising tool for imaging of exercise-induced cellular proliferation in musculo-tendinous tissue.
| Original language | English |
|---|---|
| Journal | Molecular Imaging and Biology |
| Volume | 12 |
| Issue number | 6 |
| Pages (from-to) | 626-34 |
| Number of pages | 9 |
| ISSN | 1536-1632 |
| DOIs | |
| Publication status | Published - Dec 2010 |
Keywords
- Animals
- Cell Proliferation
- Dideoxynucleosides
- Exercise Test
- Hyperplasia
- Ki-67 Antigen
- Male
- Muscle, Skeletal
- Physical Conditioning, Animal
- Positron-Emission Tomography
- Rats
- Rats, Wistar
- Running
- Tendons
- Tomography, Emission-Computed
- Up-Regulation