In vivo expression of a group I intron HEG from the antisense strand of Didymium ribosomal DNA

TY - JOUR

T1 - In vivo expression of a group I intron HEG from the antisense strand of Didymium ribosomal DNA

AU - Johansen, Steinar D

AU - Vader, Anna

AU - Sjøttem, Eva

AU - Nielsen, Henrik

N1 - Keywords: Amino Acid Sequence; Animals; Base Sequence; DNA, Antisense; DNA, Ribosomal; Endodeoxyribonucleases; Introns; Molecular Sequence Data; Physarida

PY - 2006

Y1 - 2006

N2 - Two different isolates of the myxomycete Didymium iridis harbour homing endonuclease genes that are expressed from group I introns inserted into identical sites within the small subunit ribosomal DNA. The homing endonuclease proteins are related in sequence, and their gene structures share similar features such as the presence of small spliceosomal introns and functional polyadenylation sites. However, they are transcribed from opposite strands of the ribosomal DNA and presumable by different RNA polymerases. We have previously described the in vivo expression of the I-DirI homing endonuclease from within the ribosomal RNA precursor. In this paper, we demonstrate the in vivo expression of the I-DirII homing endonuclease from the opposite strand of the Didymium rRNA gene. A comparison of the expression strategies of the two genes demonstrates the feasibility of antisense expression and provides insight into nucleolar gene expression.

AB - Two different isolates of the myxomycete Didymium iridis harbour homing endonuclease genes that are expressed from group I introns inserted into identical sites within the small subunit ribosomal DNA. The homing endonuclease proteins are related in sequence, and their gene structures share similar features such as the presence of small spliceosomal introns and functional polyadenylation sites. However, they are transcribed from opposite strands of the ribosomal DNA and presumable by different RNA polymerases. We have previously described the in vivo expression of the I-DirI homing endonuclease from within the ribosomal RNA precursor. In this paper, we demonstrate the in vivo expression of the I-DirII homing endonuclease from the opposite strand of the Didymium rRNA gene. A comparison of the expression strategies of the two genes demonstrates the feasibility of antisense expression and provides insight into nucleolar gene expression.

M3 - Journal article

C2 - 17361110

SN - 1547-6286

VL - 3

SP - 157

EP - 162

JO - R N A Biology

JF - R N A Biology

IS - 4

ER -