In-gel digestion for mass spectrometric characterization of proteins and proteomes

TY - JOUR

T1 - In-gel digestion for mass spectrometric characterization of proteins and proteomes

AU - Shevchenko, Andrej

AU - Tomas, Henrik

AU - Havlis, Jan

AU - Olsen, Jesper Velgaard

AU - Mann, Matthias

N1 - Keywords: Electrophoresis; Mass Spectrometry; Proteins; Proteomics

PY - 2006

Y1 - 2006

N2 - In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been optimized to increase the speed and sensitivity of analysis. The protocol is for the in-gel digestion of both silver and Coomassie-stained protein spots or bands and can be followed by MALDI-MS or LC-MS/MS analysis to identify proteins at sensitivities better than a few femtomoles of protein starting material.

AB - In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been optimized to increase the speed and sensitivity of analysis. The protocol is for the in-gel digestion of both silver and Coomassie-stained protein spots or bands and can be followed by MALDI-MS or LC-MS/MS analysis to identify proteins at sensitivities better than a few femtomoles of protein starting material.

U2 - 10.1038/nprot.2006.468

DO - 10.1038/nprot.2006.468

M3 - Journal article

C2 - 17406544

SN - 1754-2189

VL - 1

SP - 2856

EP - 2860

JO - Nature Protocols

JF - Nature Protocols

IS - 6

ER -