@article{d7a01dc7dcc24301893450b8555342aa,
title = "In-gel digestion for mass spectrometric characterization of proteins and proteomes",
abstract = "In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been optimized to increase the speed and sensitivity of analysis. The protocol is for the in-gel digestion of both silver and Coomassie-stained protein spots or bands and can be followed by MALDI-MS or LC-MS/MS analysis to identify proteins at sensitivities better than a few femtomoles of protein starting material.",
author = "Andrej Shevchenko and Henrik Tomas and Jan Havlis and Olsen, {Jesper Velgaard} and Matthias Mann",
note = "Keywords: Electrophoresis; Mass Spectrometry; Proteins; Proteomics",
year = "2006",
doi = "10.1038/nprot.2006.468",
language = "English",
volume = "1",
pages = "2856--60",
journal = "Nature Protocols",
issn = "1754-2189",
publisher = "nature publishing group",
number = "6",
}