Abstract
Aim: This study investigated the effect of a 15-week life style intervention (hypocaloric diet and regular exercise) on glucose tolerance, skeletal muscle lipids and muscle metabolic adaptations in 14 female and 9 male morbidly obese subjects (age: 32.5±2.3years, body mass index: 46.1±1.9kgm -2). Method: Before and after the life style intervention, an oral glucose tolerance test was performed and a muscle biopsy was obtained in the fasted state. Maximal oxygen uptake was measured by an indirect test. Results: After the intervention, body weight was decreased (P<0.05) by 11±1%, maximal oxygen uptake increased (P<0.05) by 18±5% and glucose tolerance increased (P<0.05) by 12±3%. Muscle glycogen was significantly increased by 47±14%, but muscle ceramide and triacylglycerol content remained completely unchanged. No sex difference was observed for any of these parameters, but during submaximal exercise a marked decrease (P<0.05) of 15±2% in respiratory exchange ratio was seen only in females indicating an enhanced fat oxidation. Conclusion: Despite a marked weight loss and an improved aerobic capacity muscle ceramide and triacylglycerol remained unchanged after intensive life style intervention, and muscle lipids hence do not seem to play a major role for the improved glucose tolerance in these morbidly obese subjects. Interestingly, only the females improved fat oxidation during submaximal exercise after the intervention implying the presence of a sex-dependent response to intensive life style adaptation.
| Original language | English |
|---|---|
| Journal | Acta Physiologica (Print) |
| Volume | 201 |
| Issue number | 3 |
| Pages (from-to) | 357-64 |
| Number of pages | 8 |
| ISSN | 1748-1708 |
| DOIs | |
| Publication status | Published - 1 Mar 2011 |
Keywords
- Adult
- Ceramides
- Diet Therapy
- Exercise Therapy
- Female
- Glucose
- Glucose Tolerance Test
- Humans
- Life Style
- Male
- Muscle, Skeletal
- Obesity, Morbid
- Treatment Outcome
- Triglycerides
- Young Adult
