Improved glucose tolerance after intensive life style intervention occurs without changes in muscle ceramide or triacylglycerol in morbidly obese subjects

TY - JOUR

T1 - Improved glucose tolerance after intensive life style intervention occurs without changes in muscle ceramide or triacylglycerol in morbidly obese subjects

AU - Helge, Jørn Wulff

AU - Stallknecht, Bente Merete

AU - Drachmann, Tue

AU - Hellgren, Lars

AU - Jiménez-Jiménez, R

AU - Andersen, J L

AU - Richelsen, Bjørn

AU - Bruun, J M

N1 - © 2010 The Authors. Acta Physiologica © 2010 Scandinavian Physiological Society.

PY - 2011/3/1

Y1 - 2011/3/1

N2 - Aim: This study investigated the effect of a 15-week life style intervention (hypocaloric diet and regular exercise) on glucose tolerance, skeletal muscle lipids and muscle metabolic adaptations in 14 female and 9 male morbidly obese subjects (age: 32.5±2.3years, body mass index: 46.1±1.9kgm -2). Method: Before and after the life style intervention, an oral glucose tolerance test was performed and a muscle biopsy was obtained in the fasted state. Maximal oxygen uptake was measured by an indirect test. Results: After the intervention, body weight was decreased (P<0.05) by 11±1%, maximal oxygen uptake increased (P<0.05) by 18±5% and glucose tolerance increased (P<0.05) by 12±3%. Muscle glycogen was significantly increased by 47±14%, but muscle ceramide and triacylglycerol content remained completely unchanged. No sex difference was observed for any of these parameters, but during submaximal exercise a marked decrease (P<0.05) of 15±2% in respiratory exchange ratio was seen only in females indicating an enhanced fat oxidation. Conclusion: Despite a marked weight loss and an improved aerobic capacity muscle ceramide and triacylglycerol remained unchanged after intensive life style intervention, and muscle lipids hence do not seem to play a major role for the improved glucose tolerance in these morbidly obese subjects. Interestingly, only the females improved fat oxidation during submaximal exercise after the intervention implying the presence of a sex-dependent response to intensive life style adaptation.

AB - Aim: This study investigated the effect of a 15-week life style intervention (hypocaloric diet and regular exercise) on glucose tolerance, skeletal muscle lipids and muscle metabolic adaptations in 14 female and 9 male morbidly obese subjects (age: 32.5±2.3years, body mass index: 46.1±1.9kgm -2). Method: Before and after the life style intervention, an oral glucose tolerance test was performed and a muscle biopsy was obtained in the fasted state. Maximal oxygen uptake was measured by an indirect test. Results: After the intervention, body weight was decreased (P<0.05) by 11±1%, maximal oxygen uptake increased (P<0.05) by 18±5% and glucose tolerance increased (P<0.05) by 12±3%. Muscle glycogen was significantly increased by 47±14%, but muscle ceramide and triacylglycerol content remained completely unchanged. No sex difference was observed for any of these parameters, but during submaximal exercise a marked decrease (P<0.05) of 15±2% in respiratory exchange ratio was seen only in females indicating an enhanced fat oxidation. Conclusion: Despite a marked weight loss and an improved aerobic capacity muscle ceramide and triacylglycerol remained unchanged after intensive life style intervention, and muscle lipids hence do not seem to play a major role for the improved glucose tolerance in these morbidly obese subjects. Interestingly, only the females improved fat oxidation during submaximal exercise after the intervention implying the presence of a sex-dependent response to intensive life style adaptation.

KW - Adult

KW - Ceramides

KW - Diet Therapy

KW - Exercise Therapy

KW - Female

KW - Glucose

KW - Glucose Tolerance Test

KW - Humans

KW - Life Style

KW - Male

KW - Muscle, Skeletal

KW - Obesity, Morbid

KW - Treatment Outcome

KW - Triglycerides

KW - Young Adult

U2 - 10.1111/j.1748-1716.2010.02180.x

DO - 10.1111/j.1748-1716.2010.02180.x

M3 - Journal article

C2 - 20726847

SN - 1748-1708

VL - 201

SP - 357

EP - 364

JO - Acta Physiologica (Print)

JF - Acta Physiologica (Print)

IS - 3

ER -