Abstract
An assay was designed for typing 29 of the 52 SNPs in the SNPforID multiplex on the Sequenom® MassARRAY® analyzer 4 system. The dedicated TYPER 4 software was used for automatic allele calling. The sensitivity of the assay was tested using 0.5-40ng pristine template DNA in the PCR reaction. Overall, no difference was observed in the sensitivity, measured as call rate, for the different amounts of template DNA. Typing of paternity case work samples resulted in lower call rates (54%) as compared to the sensitivity test (89%), and more incorrect calls, 0.4% as compared to 0.1%. Finally, the ability of the software to detect 1:1, 1:3 and 1:5 DNA mixtures was investigated. Compared to the sensitivity test, the call rates of the 1:1 and 1:3 mixtures were low (64-74%), whereas the call rates of the 1:5 mixtures were similar (88-90%). However, in comparison with the case work samples, the call rates of the mixtures were not unusual and mixtures may be overlooked.
| Original language | English |
|---|---|
| Journal | Forensic Science International: Genetics |
| Volume | 3 |
| Issue number | 1 |
| Pages (from-to) | e496-e497 |
| Number of pages | 2 |
| ISSN | 1872-4973 |
| Publication status | Published - Dec 2011 |