TY - JOUR
T1 - Impaired virus control and severe CD8+ T-cell-mediated immunopathology in chimeric mice deficient in gamma interferon receptor expression on both parenchymal and hematopoietic cells
AU - Henrichsen, Pernille
AU - Bartholdy, Christina
AU - Christensen, Jan Pravsgaard
AU - Thomsen, Allan Randrup
N1 - Keywords: Animals; Arenaviridae Infections; Bone Marrow Cells; CD8-Positive T-Lymphocytes; Chimera; Liver; Lymphocytic choriomeningitis virus; Mice; Mice, Inbred C57BL; Mice, Knockout; Receptors, Interferon; Spleen; Wasting Syndrome
PY - 2005
Y1 - 2005
N2 - Bone marrow chimeras were used to determine the cellular target(s) for the antiviral activity of gamma interferon (IFN-gamma). By transfusing such mice with high numbers of naive virus-specific CD8(+) T cells, a system was created in which the majority of virus-specific CD8(+) T cells would be capable of responding to IFN-gamma, but expression of the relevant receptor on non-T cells could be experimentally controlled. Only when the IFN-gamma receptor is absent on both radioresistant parenchymal and bone marrow-derived cells will chimeric mice challenged with a highly invasive, noncytolytic virus completely lack the ability to control the infection and develop severe wasting disease. Further, the study shows that IFN-gamma receptor expression on parenchymal cells in the viscera is more important for virus control than IFN-gamma receptor expression on bone marrow-derived cells.
AB - Bone marrow chimeras were used to determine the cellular target(s) for the antiviral activity of gamma interferon (IFN-gamma). By transfusing such mice with high numbers of naive virus-specific CD8(+) T cells, a system was created in which the majority of virus-specific CD8(+) T cells would be capable of responding to IFN-gamma, but expression of the relevant receptor on non-T cells could be experimentally controlled. Only when the IFN-gamma receptor is absent on both radioresistant parenchymal and bone marrow-derived cells will chimeric mice challenged with a highly invasive, noncytolytic virus completely lack the ability to control the infection and develop severe wasting disease. Further, the study shows that IFN-gamma receptor expression on parenchymal cells in the viscera is more important for virus control than IFN-gamma receptor expression on bone marrow-derived cells.
U2 - 10.1128/JVI.79.15.10073-10076.2005
DO - 10.1128/JVI.79.15.10073-10076.2005
M3 - Journal article
C2 - 16014969
SN - 0022-538X
VL - 79
SP - 10073
EP - 10076
JO - Journal of Virology
JF - Journal of Virology
IS - 15
ER -