Impaired hapten sensitization in patients with autoimmune disease

N Bangsgaard; K Engkilde; T Menné; M Løvendorf; G K Jacobsen; J Olsen; L Skov

doi:10.1111/j.1365-2249.2011.04428.x

Impaired hapten sensitization in patients with autoimmune disease

N Bangsgaard, K Engkilde, T Menné, M Løvendorf, G K Jacobsen, J Olsen, L Skov

5 Citations (Scopus)

Abstract

An inverse relation between contact allergy and autoimmune diseases is suggested from epidemiological studies. The aim of this study was to investigate susceptibility and reactivity in patients with psoriasis, patients with diabetes and healthy controls in an experimental sensitization study. We sensitized 68 adult individuals (23 patients with psoriasis, 22 patients with diabetes and 23 healthy controls) with diphenylcyclopropenone (DPCP) and assessed challenge responses with visual scoring and ultrasound. Skin biopsies from challenged skin were investigated for differences in down-regulatory mechanisms with immunohistochemistry and gene-expression profiles using microarray technology. The sensitization ratios were 26%, 36% and 65% for the psoriatic, diabetic and healthy groups, respectively. Logistic regression analysis gave an odds ratio (OR) for a patient with psoriasis or diabetes type I of being sensitized to 0·18 [95% confidence interval (CI): 0·039-0·85], P = 0·031 and 0·74 (95% CI: 0·548-1·008), P = 0·056, respectively. A high degree of forkhead box P3-positive (FoxP3(+) ) cells were found in biopsies of positively challenged reactions, but only limited numbers in negatively challenged reactions, with no difference among the groups. No specific mRNA expression was found in the challenged skin of negative elicitation reactions, also indicating no sign of active down-regulation. The study contibutes strongly to the evidence of a decreased susceptibility to develop contact allergy in individuals with autoimmune diseases such as psoriasis.

Original language	English
Journal	Clinical and Experimental Immunology
Volume	165
Issue number	3
Pages (from-to)	310-7
Number of pages	8
ISSN	0009-9104
DOIs	https://doi.org/10.1111/j.1365-2249.2011.04428.x
Publication status	Published - Sept 2011

Keywords

Adult
Autoimmune Diseases
Biopsy
Cyclopropanes
Dermatitis, Allergic Contact
Dermis
Diabetes Mellitus, Type 1
Down-Regulation
Epidermis
Female
Gene Expression
Gene Expression Profiling
Haptens
Humans
Immunization
Lymphocytes
Male
Middle Aged
Odds Ratio
Oligonucleotide Array Sequence Analysis
Principal Component Analysis
Psoriasis
Skin Tests
Up-Regulation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/j.1365-2249.2011.04428.x

https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1365-2249.2011.04428.x

Cite this

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title = "Impaired hapten sensitization in patients with autoimmune disease",

abstract = "An inverse relation between contact allergy and autoimmune diseases is suggested from epidemiological studies. The aim of this study was to investigate susceptibility and reactivity in patients with psoriasis, patients with diabetes and healthy controls in an experimental sensitization study. We sensitized 68 adult individuals (23 patients with psoriasis, 22 patients with diabetes and 23 healthy controls) with diphenylcyclopropenone (DPCP) and assessed challenge responses with visual scoring and ultrasound. Skin biopsies from challenged skin were investigated for differences in down-regulatory mechanisms with immunohistochemistry and gene-expression profiles using microarray technology. The sensitization ratios were 26%, 36% and 65% for the psoriatic, diabetic and healthy groups, respectively. Logistic regression analysis gave an odds ratio (OR) for a patient with psoriasis or diabetes type I of being sensitized to 0·18 [95% confidence interval (CI): 0·039-0·85], P = 0·031 and 0·74 (95% CI: 0·548-1·008), P = 0·056, respectively. A high degree of forkhead box P3-positive (FoxP3(+) ) cells were found in biopsies of positively challenged reactions, but only limited numbers in negatively challenged reactions, with no difference among the groups. No specific mRNA expression was found in the challenged skin of negative elicitation reactions, also indicating no sign of active down-regulation. The study contibutes strongly to the evidence of a decreased susceptibility to develop contact allergy in individuals with autoimmune diseases such as psoriasis.",

keywords = "Adult, Autoimmune Diseases, Biopsy, Cyclopropanes, Dermatitis, Allergic Contact, Dermis, Diabetes Mellitus, Type 1, Down-Regulation, Epidermis, Female, Gene Expression, Gene Expression Profiling, Haptens, Humans, Immunization, Lymphocytes, Male, Middle Aged, Odds Ratio, Oligonucleotide Array Sequence Analysis, Principal Component Analysis, Psoriasis, Skin Tests, Up-Regulation",

author = "N Bangsgaard and K Engkilde and T Menn{\'e} and M L{\o}vendorf and Jacobsen, {G K} and J Olsen and L Skov",

note = "{\textcopyright} 2011 The Authors. Clinical and Experimental Immunology {\textcopyright} 2011 British Society for Immunology.",

year = "2011",

month = sep,

doi = "10.1111/j.1365-2249.2011.04428.x",

language = "English",

volume = "165",

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journal = "Clinical and Experimental Immunology, Supplement",

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T1 - Impaired hapten sensitization in patients with autoimmune disease

AU - Bangsgaard, N

AU - Engkilde, K

AU - Menné, T

AU - Løvendorf, M

AU - Jacobsen, G K

AU - Olsen, J

AU - Skov, L

PY - 2011/9

Y1 - 2011/9

N2 - An inverse relation between contact allergy and autoimmune diseases is suggested from epidemiological studies. The aim of this study was to investigate susceptibility and reactivity in patients with psoriasis, patients with diabetes and healthy controls in an experimental sensitization study. We sensitized 68 adult individuals (23 patients with psoriasis, 22 patients with diabetes and 23 healthy controls) with diphenylcyclopropenone (DPCP) and assessed challenge responses with visual scoring and ultrasound. Skin biopsies from challenged skin were investigated for differences in down-regulatory mechanisms with immunohistochemistry and gene-expression profiles using microarray technology. The sensitization ratios were 26%, 36% and 65% for the psoriatic, diabetic and healthy groups, respectively. Logistic regression analysis gave an odds ratio (OR) for a patient with psoriasis or diabetes type I of being sensitized to 0·18 [95% confidence interval (CI): 0·039-0·85], P = 0·031 and 0·74 (95% CI: 0·548-1·008), P = 0·056, respectively. A high degree of forkhead box P3-positive (FoxP3(+) ) cells were found in biopsies of positively challenged reactions, but only limited numbers in negatively challenged reactions, with no difference among the groups. No specific mRNA expression was found in the challenged skin of negative elicitation reactions, also indicating no sign of active down-regulation. The study contibutes strongly to the evidence of a decreased susceptibility to develop contact allergy in individuals with autoimmune diseases such as psoriasis.

AB - An inverse relation between contact allergy and autoimmune diseases is suggested from epidemiological studies. The aim of this study was to investigate susceptibility and reactivity in patients with psoriasis, patients with diabetes and healthy controls in an experimental sensitization study. We sensitized 68 adult individuals (23 patients with psoriasis, 22 patients with diabetes and 23 healthy controls) with diphenylcyclopropenone (DPCP) and assessed challenge responses with visual scoring and ultrasound. Skin biopsies from challenged skin were investigated for differences in down-regulatory mechanisms with immunohistochemistry and gene-expression profiles using microarray technology. The sensitization ratios were 26%, 36% and 65% for the psoriatic, diabetic and healthy groups, respectively. Logistic regression analysis gave an odds ratio (OR) for a patient with psoriasis or diabetes type I of being sensitized to 0·18 [95% confidence interval (CI): 0·039-0·85], P = 0·031 and 0·74 (95% CI: 0·548-1·008), P = 0·056, respectively. A high degree of forkhead box P3-positive (FoxP3(+) ) cells were found in biopsies of positively challenged reactions, but only limited numbers in negatively challenged reactions, with no difference among the groups. No specific mRNA expression was found in the challenged skin of negative elicitation reactions, also indicating no sign of active down-regulation. The study contibutes strongly to the evidence of a decreased susceptibility to develop contact allergy in individuals with autoimmune diseases such as psoriasis.

KW - Adult

KW - Autoimmune Diseases

KW - Biopsy

KW - Cyclopropanes

KW - Dermatitis, Allergic Contact

KW - Dermis

KW - Diabetes Mellitus, Type 1

KW - Down-Regulation

KW - Epidermis

KW - Female

KW - Gene Expression

KW - Gene Expression Profiling

KW - Haptens

KW - Humans

KW - Immunization

KW - Lymphocytes

KW - Male

KW - Middle Aged

KW - Odds Ratio

KW - Oligonucleotide Array Sequence Analysis

KW - Principal Component Analysis

KW - Psoriasis

KW - Skin Tests

KW - Up-Regulation

U2 - 10.1111/j.1365-2249.2011.04428.x

DO - 10.1111/j.1365-2249.2011.04428.x

M3 - Journal article

C2 - 21668897

SN - 0964-2536

VL - 165

SP - 310

EP - 317

JO - Clinical and Experimental Immunology, Supplement

JF - Clinical and Experimental Immunology, Supplement

IS - 3

ER -

Impaired hapten sensitization in patients with autoimmune disease

Abstract

Keywords

UN SDGs

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