Impaired blood supply in the colonic anastomosis in mice compromises healing

Hans-Christian Pommergaard; Michael Patrick Achiam; Jakob Burcharth; Jacob Rosenberg

doi:10.9738/intsurg-d-13-00191.1

Impaired blood supply in the colonic anastomosis in mice compromises healing

Hans-Christian Pommergaard, Michael Patrick Achiam, Jakob Burcharth, Jacob Rosenberg

Department of Clinical Medicine

13 Citations (Scopus)

Abstract

Colon anastomotic leakage has a multifactorial etiology and ischemia is considered one of the most important single factors. However, no existing animal models have established a direct link between ischemia and anastomotic leakage. The aim of this study was to establish a model of colon anastomotic leakage as a result of tissue ischemia. In colon anastomoses of 53 C57BL/6 mice, varying degrees of ischemia were induced. Supplying vessels were divided with bipolar coagulation in order to reduce anastomotic breaking strength and create clinical anastomotic leakage. Breaking strength of all the ischemic anastomoses were significantly lower compared with controls. Increasing ischemia resulted in higher rates of large bowel obstruction without creating anastomotic leakage. Healing was compromised as a result of impaired blood supply. However, clinical leakage was absent. Pure ischemia in otherwise healthy experimental animals may be too simple of an approach to create clinical leakage.

Original language	English
Journal	International Surgery
Volume	100
Issue number	1
Pages (from-to)	70-6
Number of pages	7
ISSN	0020-8868
DOIs	https://doi.org/10.9738/intsurg-d-13-00191.1
Publication status	Published - Jan 2015

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title = "Impaired blood supply in the colonic anastomosis in mice compromises healing",

abstract = "Colon anastomotic leakage has a multifactorial etiology and ischemia is considered one of the most important single factors. However, no existing animal models have established a direct link between ischemia and anastomotic leakage. The aim of this study was to establish a model of colon anastomotic leakage as a result of tissue ischemia. In colon anastomoses of 53 C57BL/6 mice, varying degrees of ischemia were induced. Supplying vessels were divided with bipolar coagulation in order to reduce anastomotic breaking strength and create clinical anastomotic leakage. Breaking strength of all the ischemic anastomoses were significantly lower compared with controls. Increasing ischemia resulted in higher rates of large bowel obstruction without creating anastomotic leakage. Healing was compromised as a result of impaired blood supply. However, clinical leakage was absent. Pure ischemia in otherwise healthy experimental animals may be too simple of an approach to create clinical leakage.",

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T1 - Impaired blood supply in the colonic anastomosis in mice compromises healing

AU - Pommergaard, Hans-Christian

AU - Achiam, Michael Patrick

AU - Burcharth, Jakob

AU - Rosenberg, Jacob

PY - 2015/1

Y1 - 2015/1

N2 - Colon anastomotic leakage has a multifactorial etiology and ischemia is considered one of the most important single factors. However, no existing animal models have established a direct link between ischemia and anastomotic leakage. The aim of this study was to establish a model of colon anastomotic leakage as a result of tissue ischemia. In colon anastomoses of 53 C57BL/6 mice, varying degrees of ischemia were induced. Supplying vessels were divided with bipolar coagulation in order to reduce anastomotic breaking strength and create clinical anastomotic leakage. Breaking strength of all the ischemic anastomoses were significantly lower compared with controls. Increasing ischemia resulted in higher rates of large bowel obstruction without creating anastomotic leakage. Healing was compromised as a result of impaired blood supply. However, clinical leakage was absent. Pure ischemia in otherwise healthy experimental animals may be too simple of an approach to create clinical leakage.

AB - Colon anastomotic leakage has a multifactorial etiology and ischemia is considered one of the most important single factors. However, no existing animal models have established a direct link between ischemia and anastomotic leakage. The aim of this study was to establish a model of colon anastomotic leakage as a result of tissue ischemia. In colon anastomoses of 53 C57BL/6 mice, varying degrees of ischemia were induced. Supplying vessels were divided with bipolar coagulation in order to reduce anastomotic breaking strength and create clinical anastomotic leakage. Breaking strength of all the ischemic anastomoses were significantly lower compared with controls. Increasing ischemia resulted in higher rates of large bowel obstruction without creating anastomotic leakage. Healing was compromised as a result of impaired blood supply. However, clinical leakage was absent. Pure ischemia in otherwise healthy experimental animals may be too simple of an approach to create clinical leakage.

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DO - 10.9738/intsurg-d-13-00191.1

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SN - 0020-8868

VL - 100

SP - 70

EP - 76

JO - International Surgery

JF - International Surgery

IS - 1

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Impaired blood supply in the colonic anastomosis in mice compromises healing

Abstract

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