Impaired blood supply in the colonic anastomosis in mice compromises healing

Hans-Christian Pommergaard; Michael Patrick Achiam; Jakob Burcharth; Jacob Rosenberg

doi:10.9738/intsurg-d-13-00191.1

Impaired blood supply in the colonic anastomosis in mice compromises healing

Hans-Christian Pommergaard, Michael Patrick Achiam, Jakob Burcharth, Jacob Rosenberg

Institut for Klinisk Medicin

13 Citationer (Scopus)

Abstract

Colon anastomotic leakage has a multifactorial etiology and ischemia is considered one of the most important single factors. However, no existing animal models have established a direct link between ischemia and anastomotic leakage. The aim of this study was to establish a model of colon anastomotic leakage as a result of tissue ischemia. In colon anastomoses of 53 C57BL/6 mice, varying degrees of ischemia were induced. Supplying vessels were divided with bipolar coagulation in order to reduce anastomotic breaking strength and create clinical anastomotic leakage. Breaking strength of all the ischemic anastomoses were significantly lower compared with controls. Increasing ischemia resulted in higher rates of large bowel obstruction without creating anastomotic leakage. Healing was compromised as a result of impaired blood supply. However, clinical leakage was absent. Pure ischemia in otherwise healthy experimental animals may be too simple of an approach to create clinical leakage.

Originalsprog	Engelsk
Tidsskrift	International Surgery
Vol/bind	100
Udgave nummer	1
Sider (fra-til)	70-6
Antal sider	7
ISSN	0020-8868
DOI	https://doi.org/10.9738/intsurg-d-13-00191.1
Status	Udgivet - jan. 2015

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10.9738/intsurg-d-13-00191.1

https://meridian.allenpress.com/international-surgery/article-pdf/100/1/70/2216021/intsurg-d-13-00191_1.pdf

Citationsformater

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author = "Hans-Christian Pommergaard and Achiam, {Michael Patrick} and Jakob Burcharth and Jacob Rosenberg",

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T1 - Impaired blood supply in the colonic anastomosis in mice compromises healing

AU - Pommergaard, Hans-Christian

AU - Achiam, Michael Patrick

AU - Burcharth, Jakob

AU - Rosenberg, Jacob

PY - 2015/1

Y1 - 2015/1

N2 - Colon anastomotic leakage has a multifactorial etiology and ischemia is considered one of the most important single factors. However, no existing animal models have established a direct link between ischemia and anastomotic leakage. The aim of this study was to establish a model of colon anastomotic leakage as a result of tissue ischemia. In colon anastomoses of 53 C57BL/6 mice, varying degrees of ischemia were induced. Supplying vessels were divided with bipolar coagulation in order to reduce anastomotic breaking strength and create clinical anastomotic leakage. Breaking strength of all the ischemic anastomoses were significantly lower compared with controls. Increasing ischemia resulted in higher rates of large bowel obstruction without creating anastomotic leakage. Healing was compromised as a result of impaired blood supply. However, clinical leakage was absent. Pure ischemia in otherwise healthy experimental animals may be too simple of an approach to create clinical leakage.

AB - Colon anastomotic leakage has a multifactorial etiology and ischemia is considered one of the most important single factors. However, no existing animal models have established a direct link between ischemia and anastomotic leakage. The aim of this study was to establish a model of colon anastomotic leakage as a result of tissue ischemia. In colon anastomoses of 53 C57BL/6 mice, varying degrees of ischemia were induced. Supplying vessels were divided with bipolar coagulation in order to reduce anastomotic breaking strength and create clinical anastomotic leakage. Breaking strength of all the ischemic anastomoses were significantly lower compared with controls. Increasing ischemia resulted in higher rates of large bowel obstruction without creating anastomotic leakage. Healing was compromised as a result of impaired blood supply. However, clinical leakage was absent. Pure ischemia in otherwise healthy experimental animals may be too simple of an approach to create clinical leakage.

U2 - 10.9738/intsurg-d-13-00191.1

DO - 10.9738/intsurg-d-13-00191.1

M3 - Journal article

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SN - 0020-8868

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SP - 70

EP - 76

JO - International Surgery

JF - International Surgery

IS - 1

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Impaired blood supply in the colonic anastomosis in mice compromises healing

Abstract

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