Immunomicroscopic localization of aminopeptidase N in the pig enterocyte. Implications for the route of intracellular transport

TY - JOUR

T1 - Immunomicroscopic localization of aminopeptidase N in the pig enterocyte. Implications for the route of intracellular transport

AU - Hansen, Gert Helge

AU - Sjöström, H

AU - Norén, Ove

AU - Dabelsteen, E

N1 - Keywords: Aminopeptidases; Animals; Antigens, CD13; Epithelial Cells; Epithelium; Fluorescent Antibody Technique; Ileum; Microscopy, Electron; Swine

PY - 1987

Y1 - 1987

N2 - The subcellular localization of aminopeptidase N (EC 3.4.11.2) in the pig enterocyte was investigated by immunofluorescence and immunoelectron microscopy (immunogold staining). By indirect immunofluorescence on either frozen or paraffin-embedded sections, a very intense staining in the microvillar membrane and a weak intracellular staining was demonstrated. No staining was detected in the basolateral membrane. Likewise, the immunogold labelling on Epon-embedded sections was concentrated in the microvillar membrane, whereas the basolateral membrane did not contain significant amounts of labelling. Labelling was demonstrated in the Golgi apparatus and in a minor fraction of the intracellular smooth vesicles positioned between the Golgi apparatus and the microvillar membrane. These observations are compatible with the view that newly synthesized aminopeptidase N is delivered directly to the microvillar membrane by smooth vesicles having a diameter about 70 to 100 nm and does not pass the basolateral membrane on its way to the brush border membrane.

AB - The subcellular localization of aminopeptidase N (EC 3.4.11.2) in the pig enterocyte was investigated by immunofluorescence and immunoelectron microscopy (immunogold staining). By indirect immunofluorescence on either frozen or paraffin-embedded sections, a very intense staining in the microvillar membrane and a weak intracellular staining was demonstrated. No staining was detected in the basolateral membrane. Likewise, the immunogold labelling on Epon-embedded sections was concentrated in the microvillar membrane, whereas the basolateral membrane did not contain significant amounts of labelling. Labelling was demonstrated in the Golgi apparatus and in a minor fraction of the intracellular smooth vesicles positioned between the Golgi apparatus and the microvillar membrane. These observations are compatible with the view that newly synthesized aminopeptidase N is delivered directly to the microvillar membrane by smooth vesicles having a diameter about 70 to 100 nm and does not pass the basolateral membrane on its way to the brush border membrane.

M3 - Journal article

C2 - 2885197

SN - 0724-5130

VL - 43

SP - 253

EP - 259

JO - Cytobiologie

JF - Cytobiologie

IS - 2

ER -