@article{ed0fe020e31211ddb5fc000ea68e967b,
title = "Immunomicroscopic localization of aminopeptidase N in the pig enterocyte. Implications for the route of intracellular transport",
abstract = "The subcellular localization of aminopeptidase N (EC 3.4.11.2) in the pig enterocyte was investigated by immunofluorescence and immunoelectron microscopy (immunogold staining). By indirect immunofluorescence on either frozen or paraffin-embedded sections, a very intense staining in the microvillar membrane and a weak intracellular staining was demonstrated. No staining was detected in the basolateral membrane. Likewise, the immunogold labelling on Epon-embedded sections was concentrated in the microvillar membrane, whereas the basolateral membrane did not contain significant amounts of labelling. Labelling was demonstrated in the Golgi apparatus and in a minor fraction of the intracellular smooth vesicles positioned between the Golgi apparatus and the microvillar membrane. These observations are compatible with the view that newly synthesized aminopeptidase N is delivered directly to the microvillar membrane by smooth vesicles having a diameter about 70 to 100 nm and does not pass the basolateral membrane on its way to the brush border membrane.",
author = "Hansen, {Gert Helge} and H Sj{\"o}str{\"o}m and Ove Nor{\'e}n and E Dabelsteen",
note = "Keywords: Aminopeptidases; Animals; Antigens, CD13; Epithelial Cells; Epithelium; Fluorescent Antibody Technique; Ileum; Microscopy, Electron; Swine",
year = "1987",
language = "English",
volume = "43",
pages = "253--9",
journal = "Cytobiologie",
issn = "0724-5130",
publisher = "Elsevier GmbH - Urban und Fischer",
number = "2",
}