Immunohistochemical detection of interleukin-8 in inflamed porcine tissues

Henriette Laursen; Henrik Elvang Jensen; Páll S. Leifsson; Louise Kruse Jensen; Johanna Kristina G Christiansen; Ramona Trebbien; Ole Lerberg Nielsen

doi:10.1016/j.vetimm.2014.01.013

Immunohistochemical detection of interleukin-8 in inflamed porcine tissues

Henriette Laursen, Henrik Elvang Jensen, Páll S. Leifsson, Louise Kruse Jensen, Johanna Kristina G Christiansen, Ramona Trebbien, Ole Lerberg Nielsen

4 Citations (Scopus)

Abstract

The objective of this study was to identify the specific localization of interleukin-8 (IL-8) in cells in situ in a variety of inflammatory processes in different tissues from pigs. Our hypothesis was that IL-8 primarily is a neutrophil related cytokine present in all extravascular neutrophils while expression also occurs in other cell types in response to an inflammatory stimulus. Using IL-8 immunohistochemistry we discovered that neutrophils were the predominant IL-8 positive cell population while epithelial cell types and endothelium of postcapillary venules could be positive when situated in close vicinity of an inflammatory lesion. Furthermore, endothelial cells of newly formed vessels in granulation tissue were positive in some specimens. Some sub-populations of inflammatory neutrophils were, however, IL-8 negative which could reflect some phase of neutrophil swarming.

Original language	English
Journal	Veterinary Immunology and Immunopathology
Volume	159
Issue number	1-2
Pages (from-to)	97-102
Number of pages	6
ISSN	0165-2427
DOIs	https://doi.org/10.1016/j.vetimm.2014.01.013
Publication status	Published - 15 May 2014

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title = "Immunohistochemical detection of interleukin-8 in inflamed porcine tissues",

abstract = "The objective of this study was to identify the specific localization of interleukin-8 (IL-8) in cells in situ in a variety of inflammatory processes in different tissues from pigs. Our hypothesis was that IL-8 primarily is a neutrophil related cytokine present in all extravascular neutrophils while expression also occurs in other cell types in response to an inflammatory stimulus. Using IL-8 immunohistochemistry we discovered that neutrophils were the predominant IL-8 positive cell population while epithelial cell types and endothelium of postcapillary venules could be positive when situated in close vicinity of an inflammatory lesion. Furthermore, endothelial cells of newly formed vessels in granulation tissue were positive in some specimens. Some sub-populations of inflammatory neutrophils were, however, IL-8 negative which could reflect some phase of neutrophil swarming.",

author = "Henriette Laursen and Jensen, {Henrik Elvang} and Leifsson, {P{\'a}ll S.} and Jensen, {Louise Kruse} and Christiansen, {Johanna Kristina G} and Ramona Trebbien and Nielsen, {Ole Lerberg}",

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T1 - Immunohistochemical detection of interleukin-8 in inflamed porcine tissues

AU - Laursen, Henriette

AU - Jensen, Henrik Elvang

AU - Leifsson, Páll S.

AU - Jensen, Louise Kruse

AU - Christiansen, Johanna Kristina G

AU - Trebbien, Ramona

AU - Nielsen, Ole Lerberg

PY - 2014/5/15

Y1 - 2014/5/15

N2 - The objective of this study was to identify the specific localization of interleukin-8 (IL-8) in cells in situ in a variety of inflammatory processes in different tissues from pigs. Our hypothesis was that IL-8 primarily is a neutrophil related cytokine present in all extravascular neutrophils while expression also occurs in other cell types in response to an inflammatory stimulus. Using IL-8 immunohistochemistry we discovered that neutrophils were the predominant IL-8 positive cell population while epithelial cell types and endothelium of postcapillary venules could be positive when situated in close vicinity of an inflammatory lesion. Furthermore, endothelial cells of newly formed vessels in granulation tissue were positive in some specimens. Some sub-populations of inflammatory neutrophils were, however, IL-8 negative which could reflect some phase of neutrophil swarming.

AB - The objective of this study was to identify the specific localization of interleukin-8 (IL-8) in cells in situ in a variety of inflammatory processes in different tissues from pigs. Our hypothesis was that IL-8 primarily is a neutrophil related cytokine present in all extravascular neutrophils while expression also occurs in other cell types in response to an inflammatory stimulus. Using IL-8 immunohistochemistry we discovered that neutrophils were the predominant IL-8 positive cell population while epithelial cell types and endothelium of postcapillary venules could be positive when situated in close vicinity of an inflammatory lesion. Furthermore, endothelial cells of newly formed vessels in granulation tissue were positive in some specimens. Some sub-populations of inflammatory neutrophils were, however, IL-8 negative which could reflect some phase of neutrophil swarming.

U2 - 10.1016/j.vetimm.2014.01.013

DO - 10.1016/j.vetimm.2014.01.013

M3 - Journal article

C2 - 24698104

SN - 0165-2427

VL - 159

SP - 97

EP - 102

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

IS - 1-2

ER -

Immunohistochemical detection of interleukin-8 in inflamed porcine tissues

Abstract

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