Immune regulation by fibroblasts in tissue injury depends on uPARAP-mediated uptake of collectins

Henrik J. Jürgensen; Kirstine S. Nørregaard; Megan M. Sibree; Eric Santoni‑Rugiu; Daniel H. Madsen; Katharina Wassilew; Dorrit Krustrup; Peter Garred; Thomas H. Bugge; Lars H. Engelholm; Niels Behrendt

doi:10.1083/jcb.201802148

Immune regulation by fibroblasts in tissue injury depends on uPARAP-mediated uptake of collectins

Henrik J. Jürgensen, Kirstine S. Nørregaard, Megan M. Sibree, Eric Santoni‑Rugiu, Daniel H. Madsen, Katharina Wassilew, Dorrit Krustrup, Peter Garred, Thomas H. Bugge, Lars H. Engelholm, Niels Behrendt^*

^*Corresponding author for this work

6 Citations (Scopus)

Abstract

Collectins such as mannose-binding lectin (MBL) and surfactant protein D (SP-D) become temporarily deposited in extravascular compartments after tissue injury and perform immune-stimulatory or inflammation-limiting functions. However, their turnover mechanisms, necessary to prevent excessive tissue damage, are virtually unknown. In this study, we show that fibroblasts in injured tissues undertake the clearance of collectins by using the endocytic collagen receptor uPARAP. In cellular assays, several types of collectins were endocytosed in a highly specific uPARAP-dependent process, not shared by the closely related receptor MR/CD206. When introduced into dermis or bleomycin-injured lungs of mice, collectins MBL and SP-D were endocytosed and routed for lysosomal degradation by uPARAP-positive fibroblasts. Fibroblast-specific expression of uPARAP governed endogenous SP-D levels and overall survival after lung injury. In lung tissue from idiopathic pulmonary fibrosis patients, a strong up-regulation of uPARAP was observed in fibroblasts adjacent to regions with SP-D secretion. This study demonstrates a novel immune-regulatory function of fibroblasts and identifies uPARAP as an endocytic receptor in immunity.

Original language	English
Journal	Journal of Cell Biology
Volume	218
Issue number	1
Pages (from-to)	333-349
Number of pages	17
ISSN	0021-9525
DOIs	https://doi.org/10.1083/jcb.201802148
Publication status	Published - 1 Jan 2019

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title = "Immune regulation by fibroblasts in tissue injury depends on uPARAP-mediated uptake of collectins",

abstract = "Collectins such as mannose-binding lectin (MBL) and surfactant protein D (SP-D) become temporarily deposited in extravascular compartments after tissue injury and perform immune-stimulatory or inflammation-limiting functions. However, their turnover mechanisms, necessary to prevent excessive tissue damage, are virtually unknown. In this study, we show that fibroblasts in injured tissues undertake the clearance of collectins by using the endocytic collagen receptor uPARAP. In cellular assays, several types of collectins were endocytosed in a highly specific uPARAP-dependent process, not shared by the closely related receptor MR/CD206. When introduced into dermis or bleomycin-injured lungs of mice, collectins MBL and SP-D were endocytosed and routed for lysosomal degradation by uPARAP-positive fibroblasts. Fibroblast-specific expression of uPARAP governed endogenous SP-D levels and overall survival after lung injury. In lung tissue from idiopathic pulmonary fibrosis patients, a strong up-regulation of uPARAP was observed in fibroblasts adjacent to regions with SP-D secretion. This study demonstrates a novel immune-regulatory function of fibroblasts and identifies uPARAP as an endocytic receptor in immunity.",

author = "J{\"u}rgensen, {Henrik J.} and N{\o}rregaard, {Kirstine S.} and Sibree, {Megan M.} and Eric Santoni‑Rugiu and Madsen, {Daniel H.} and Katharina Wassilew and Dorrit Krustrup and Peter Garred and Bugge, {Thomas H.} and Engelholm, {Lars H.} and Niels Behrendt",

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AU - Jürgensen, Henrik J.

AU - Nørregaard, Kirstine S.

AU - Sibree, Megan M.

AU - Santoni‑Rugiu, Eric

AU - Madsen, Daniel H.

AU - Wassilew, Katharina

AU - Krustrup, Dorrit

AU - Garred, Peter

AU - Bugge, Thomas H.

AU - Engelholm, Lars H.

AU - Behrendt, Niels

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Collectins such as mannose-binding lectin (MBL) and surfactant protein D (SP-D) become temporarily deposited in extravascular compartments after tissue injury and perform immune-stimulatory or inflammation-limiting functions. However, their turnover mechanisms, necessary to prevent excessive tissue damage, are virtually unknown. In this study, we show that fibroblasts in injured tissues undertake the clearance of collectins by using the endocytic collagen receptor uPARAP. In cellular assays, several types of collectins were endocytosed in a highly specific uPARAP-dependent process, not shared by the closely related receptor MR/CD206. When introduced into dermis or bleomycin-injured lungs of mice, collectins MBL and SP-D were endocytosed and routed for lysosomal degradation by uPARAP-positive fibroblasts. Fibroblast-specific expression of uPARAP governed endogenous SP-D levels and overall survival after lung injury. In lung tissue from idiopathic pulmonary fibrosis patients, a strong up-regulation of uPARAP was observed in fibroblasts adjacent to regions with SP-D secretion. This study demonstrates a novel immune-regulatory function of fibroblasts and identifies uPARAP as an endocytic receptor in immunity.

AB - Collectins such as mannose-binding lectin (MBL) and surfactant protein D (SP-D) become temporarily deposited in extravascular compartments after tissue injury and perform immune-stimulatory or inflammation-limiting functions. However, their turnover mechanisms, necessary to prevent excessive tissue damage, are virtually unknown. In this study, we show that fibroblasts in injured tissues undertake the clearance of collectins by using the endocytic collagen receptor uPARAP. In cellular assays, several types of collectins were endocytosed in a highly specific uPARAP-dependent process, not shared by the closely related receptor MR/CD206. When introduced into dermis or bleomycin-injured lungs of mice, collectins MBL and SP-D were endocytosed and routed for lysosomal degradation by uPARAP-positive fibroblasts. Fibroblast-specific expression of uPARAP governed endogenous SP-D levels and overall survival after lung injury. In lung tissue from idiopathic pulmonary fibrosis patients, a strong up-regulation of uPARAP was observed in fibroblasts adjacent to regions with SP-D secretion. This study demonstrates a novel immune-regulatory function of fibroblasts and identifies uPARAP as an endocytic receptor in immunity.

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DO - 10.1083/jcb.201802148

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Immune regulation by fibroblasts in tissue injury depends on uPARAP-mediated uptake of collectins

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