Identifying sources and estimating glandular output of salivary TIMP-1

Lars Holten-Andersen; Siri Beier Jensen; Allan Bardow Jensen; Jakob Le Fèvre Harslund; M. Thaysen-Andersen; Ulrik Axel Lademann; Pernille Autzen Usher; Hanne Kjær Offenberg; Peter Højrup; Jesper Reibel; H.J. Nielsen; Nils Brunner; Birgitte Nauntofte; L Holten-Andersen; S Beier Jensen; A Bardow; J Harslund; M Thaysen-Andersen; U Lademann; P Autzen Usher; H Offenberg; P Højrup; J Reibel; Hans Jørgen Nielsen; N Brunner; B Nauntofte

doi:10.1080/00365510701883180

Identifying sources and estimating glandular output of salivary TIMP-1

Lars Holten-Andersen, Siri Beier Jensen, Allan Bardow Jensen, Jakob Le Fèvre Harslund, M. Thaysen-Andersen, Ulrik Axel Lademann, Pernille Autzen Usher, Hanne Kjær Offenberg, Peter Højrup, Jesper Reibel, H.J. Nielsen, Nils Brunner, Birgitte Nauntofte, L Holten-Andersen, S Beier Jensen, A Bardow, J Harslund, M Thaysen-Andersen, U Lademann, P Autzen UsherH Offenberg, P Højrup, J Reibel, Hans Jørgen Nielsen, N Brunner, B Nauntofte

Department of Clinical Medicine

5 Citations (Scopus)

Abstract

Objective. Tissue inhibitor of metalloproteinases 1 (TIMP-1) has been identified as a potential biomarker in diseases such as cancer, cardiovascular diseases and diabetes. Since TIMP-1 resides in most tissues and bodily fluids, we evaluated the potential of using saliva to obtain reproducible TIMP-1 measurements in a non-invasive manner. Material and methods. Samples of unstimulated and stimulated whole saliva and saliva collected from individual glands were analysed for TIMP-1 content. A TIMP-1 ELISA was validated for use in saliva testing and the most optimal sampling and handling procedures for reproducible measurements identified. Western blotting and MALDI-TOF mass spectrometry were used for confirmatory analyses. Results. The TIMP-1 ELISA was found suitable for saliva measurements. All saliva secretions contained TIMP-1, but in different concentrations ranging from 2.81 ng/mL in submandibular/sublingual saliva to 173.88 ng/mL in parotid saliva. TIMP-1 concentrations were influenced to a varying degree by fluctuations in flow. We found the lowest output in submandibular/sublingual saliva stimulated with 0.5 % citric acid (3.56 ng/min) and highest output in chewing-stimulated whole saliva (267.01 ng/min). Conclusion. This study shows that saliva contains authentic TIMP-1, the concentration of which was found to depend on gland type and salivary flow. Stimulated whole saliva is suggested as a reliable and easily accessible source for TIMP-1 determinations in bodily fluids.

Original language	English
Journal	Scandinavian Journal of Clinical & Laboratory Investigation
Volume	68
Issue number	7
Pages (from-to)	548-54
Number of pages	6
ISSN	0036-5513
DOIs	https://doi.org/10.1080/00365510701883180 https://doi.org/10.1080/00365510701883180
Publication status	Published - 2008

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Holten-Andersen, L., Jensen, S. B., Jensen, A. B., Harslund, J. L. F., Thaysen-Andersen, M., Lademann, U. A., Usher, P. A., Offenberg, H. K., Højrup, P., Reibel, J., Nielsen, H. J., Brunner, N., Nauntofte, B., Holten-Andersen, L., Beier Jensen, S., Bardow, A., Harslund, J., Thaysen-Andersen, M., Lademann, U., ... Nauntofte, B. (2008). Identifying sources and estimating glandular output of salivary TIMP-1. Scandinavian Journal of Clinical & Laboratory Investigation, 68(7), 548-54. https://doi.org/10.1080/00365510701883180, https://doi.org/10.1080/00365510701883180

Holten-Andersen, L, Jensen, SB, Jensen, AB, Harslund, JLF, Thaysen-Andersen, M, Lademann, UA, Usher, PA, Offenberg, HK, Højrup, P, Reibel, J, Nielsen, HJ, Brunner, N, Nauntofte, B, Holten-Andersen, L, Beier Jensen, S, Bardow, A, Harslund, J, Thaysen-Andersen, M, Lademann, U, Autzen Usher, P, Offenberg, H, Højrup, P, Reibel, J, Nielsen, HJ, Brunner, N & Nauntofte, B 2008, 'Identifying sources and estimating glandular output of salivary TIMP-1', Scandinavian Journal of Clinical & Laboratory Investigation, vol. 68, no. 7, pp. 548-54. https://doi.org/10.1080/00365510701883180, https://doi.org/10.1080/00365510701883180

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title = "Identifying sources and estimating glandular output of salivary TIMP-1",

abstract = "Objective. Tissue inhibitor of metalloproteinases 1 (TIMP-1) has been identified as a potential biomarker in diseases such as cancer, cardiovascular diseases and diabetes. Since TIMP-1 resides in most tissues and bodily fluids, we evaluated the potential of using saliva to obtain reproducible TIMP-1 measurements in a non-invasive manner. Material and methods. Samples of unstimulated and stimulated whole saliva and saliva collected from individual glands were analysed for TIMP-1 content. A TIMP-1 ELISA was validated for use in saliva testing and the most optimal sampling and handling procedures for reproducible measurements identified. Western blotting and MALDI-TOF mass spectrometry were used for confirmatory analyses. Results. The TIMP-1 ELISA was found suitable for saliva measurements. All saliva secretions contained TIMP-1, but in different concentrations ranging from 2.81 ng/mL in submandibular/sublingual saliva to 173.88 ng/mL in parotid saliva. TIMP-1 concentrations were influenced to a varying degree by fluctuations in flow. We found the lowest output in submandibular/sublingual saliva stimulated with 0.5 % citric acid (3.56 ng/min) and highest output in chewing-stimulated whole saliva (267.01 ng/min). Conclusion. This study shows that saliva contains authentic TIMP-1, the concentration of which was found to depend on gland type and salivary flow. Stimulated whole saliva is suggested as a reliable and easily accessible source for TIMP-1 determinations in bodily fluids.",

author = "Lars Holten-Andersen and Jensen, {Siri Beier} and Jensen, {Allan Bardow} and Harslund, {Jakob Le F{\`e}vre} and M. Thaysen-Andersen and Lademann, {Ulrik Axel} and Usher, {Pernille Autzen} and Offenberg, {Hanne Kj{\ae}r} and Peter H{\o}jrup and Jesper Reibel and H.J. Nielsen and Nils Brunner and Birgitte Nauntofte and L Holten-Andersen and {Beier Jensen}, S and A Bardow and J Harslund and M Thaysen-Andersen and U Lademann and {Autzen Usher}, P and H Offenberg and P H{\o}jrup and J Reibel and Nielsen, {Hans J{\o}rgen} and N Brunner and B Nauntofte",

year = "2008",

doi = "10.1080/00365510701883180",

language = "English",

volume = "68",

pages = "548--54",

journal = "Scandinavian Journal of Clinical & Laboratory Investigation",

issn = "0036-5513",

publisher = "Taylor & Francis",

number = "7",

}

TY - JOUR

T1 - Identifying sources and estimating glandular output of salivary TIMP-1

AU - Holten-Andersen, Lars

AU - Jensen, Siri Beier

AU - Jensen, Allan Bardow

AU - Harslund, Jakob Le Fèvre

AU - Thaysen-Andersen, M.

AU - Lademann, Ulrik Axel

AU - Usher, Pernille Autzen

AU - Offenberg, Hanne Kjær

AU - Højrup, Peter

AU - Reibel, Jesper

AU - Nielsen, H.J.

AU - Brunner, Nils

AU - Nauntofte, Birgitte

AU - Holten-Andersen, L

AU - Beier Jensen, S

AU - Bardow, A

AU - Harslund, J

AU - Thaysen-Andersen, M

AU - Lademann, U

AU - Autzen Usher, P

AU - Offenberg, H

AU - Højrup, P

AU - Reibel, J

AU - Nielsen, Hans Jørgen

AU - Brunner, N

AU - Nauntofte, B

PY - 2008

Y1 - 2008

N2 - Objective. Tissue inhibitor of metalloproteinases 1 (TIMP-1) has been identified as a potential biomarker in diseases such as cancer, cardiovascular diseases and diabetes. Since TIMP-1 resides in most tissues and bodily fluids, we evaluated the potential of using saliva to obtain reproducible TIMP-1 measurements in a non-invasive manner. Material and methods. Samples of unstimulated and stimulated whole saliva and saliva collected from individual glands were analysed for TIMP-1 content. A TIMP-1 ELISA was validated for use in saliva testing and the most optimal sampling and handling procedures for reproducible measurements identified. Western blotting and MALDI-TOF mass spectrometry were used for confirmatory analyses. Results. The TIMP-1 ELISA was found suitable for saliva measurements. All saliva secretions contained TIMP-1, but in different concentrations ranging from 2.81 ng/mL in submandibular/sublingual saliva to 173.88 ng/mL in parotid saliva. TIMP-1 concentrations were influenced to a varying degree by fluctuations in flow. We found the lowest output in submandibular/sublingual saliva stimulated with 0.5 % citric acid (3.56 ng/min) and highest output in chewing-stimulated whole saliva (267.01 ng/min). Conclusion. This study shows that saliva contains authentic TIMP-1, the concentration of which was found to depend on gland type and salivary flow. Stimulated whole saliva is suggested as a reliable and easily accessible source for TIMP-1 determinations in bodily fluids.

AB - Objective. Tissue inhibitor of metalloproteinases 1 (TIMP-1) has been identified as a potential biomarker in diseases such as cancer, cardiovascular diseases and diabetes. Since TIMP-1 resides in most tissues and bodily fluids, we evaluated the potential of using saliva to obtain reproducible TIMP-1 measurements in a non-invasive manner. Material and methods. Samples of unstimulated and stimulated whole saliva and saliva collected from individual glands were analysed for TIMP-1 content. A TIMP-1 ELISA was validated for use in saliva testing and the most optimal sampling and handling procedures for reproducible measurements identified. Western blotting and MALDI-TOF mass spectrometry were used for confirmatory analyses. Results. The TIMP-1 ELISA was found suitable for saliva measurements. All saliva secretions contained TIMP-1, but in different concentrations ranging from 2.81 ng/mL in submandibular/sublingual saliva to 173.88 ng/mL in parotid saliva. TIMP-1 concentrations were influenced to a varying degree by fluctuations in flow. We found the lowest output in submandibular/sublingual saliva stimulated with 0.5 % citric acid (3.56 ng/min) and highest output in chewing-stimulated whole saliva (267.01 ng/min). Conclusion. This study shows that saliva contains authentic TIMP-1, the concentration of which was found to depend on gland type and salivary flow. Stimulated whole saliva is suggested as a reliable and easily accessible source for TIMP-1 determinations in bodily fluids.

U2 - 10.1080/00365510701883180

DO - 10.1080/00365510701883180

M3 - Journal article

C2 - 18609089

SN - 0036-5513

VL - 68

SP - 548

EP - 554

JO - Scandinavian Journal of Clinical & Laboratory Investigation

JF - Scandinavian Journal of Clinical & Laboratory Investigation

IS - 7

ER -

Identifying sources and estimating glandular output of salivary TIMP-1

Abstract

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