TY - JOUR
T1 - Identifying sources and estimating glandular output of salivary TIMP-1
AU - Holten-Andersen, Lars
AU - Jensen, Siri Beier
AU - Jensen, Allan Bardow
AU - Harslund, Jakob Le Fèvre
AU - Thaysen-Andersen, M.
AU - Lademann, Ulrik Axel
AU - Usher, Pernille Autzen
AU - Offenberg, Hanne Kjær
AU - Højrup, Peter
AU - Reibel, Jesper
AU - Nielsen, H.J.
AU - Brunner, Nils
AU - Nauntofte, Birgitte
AU - Holten-Andersen, L
AU - Beier Jensen, S
AU - Bardow, A
AU - Harslund, J
AU - Thaysen-Andersen, M
AU - Lademann, U
AU - Autzen Usher, P
AU - Offenberg, H
AU - Højrup, P
AU - Reibel, J
AU - Nielsen, Hans Jørgen
AU - Brunner, N
AU - Nauntofte, B
PY - 2008
Y1 - 2008
N2 - Objective. Tissue inhibitor of metalloproteinases 1 (TIMP-1) has been identified as a potential biomarker in diseases such as cancer, cardiovascular diseases and diabetes. Since TIMP-1 resides in most tissues and bodily fluids, we evaluated the potential of using saliva to obtain reproducible TIMP-1 measurements in a non-invasive manner. Material and methods. Samples of unstimulated and stimulated whole saliva and saliva collected from individual glands were analysed for TIMP-1 content. A TIMP-1 ELISA was validated for use in saliva testing and the most optimal sampling and handling procedures for reproducible measurements identified. Western blotting and MALDI-TOF mass spectrometry were used for confirmatory analyses. Results. The TIMP-1 ELISA was found suitable for saliva measurements. All saliva secretions contained TIMP-1, but in different concentrations ranging from 2.81 ng/mL in submandibular/sublingual saliva to 173.88 ng/mL in parotid saliva. TIMP-1 concentrations were influenced to a varying degree by fluctuations in flow. We found the lowest output in submandibular/sublingual saliva stimulated with 0.5 % citric acid (3.56 ng/min) and highest output in chewing-stimulated whole saliva (267.01 ng/min). Conclusion. This study shows that saliva contains authentic TIMP-1, the concentration of which was found to depend on gland type and salivary flow. Stimulated whole saliva is suggested as a reliable and easily accessible source for TIMP-1 determinations in bodily fluids.
AB - Objective. Tissue inhibitor of metalloproteinases 1 (TIMP-1) has been identified as a potential biomarker in diseases such as cancer, cardiovascular diseases and diabetes. Since TIMP-1 resides in most tissues and bodily fluids, we evaluated the potential of using saliva to obtain reproducible TIMP-1 measurements in a non-invasive manner. Material and methods. Samples of unstimulated and stimulated whole saliva and saliva collected from individual glands were analysed for TIMP-1 content. A TIMP-1 ELISA was validated for use in saliva testing and the most optimal sampling and handling procedures for reproducible measurements identified. Western blotting and MALDI-TOF mass spectrometry were used for confirmatory analyses. Results. The TIMP-1 ELISA was found suitable for saliva measurements. All saliva secretions contained TIMP-1, but in different concentrations ranging from 2.81 ng/mL in submandibular/sublingual saliva to 173.88 ng/mL in parotid saliva. TIMP-1 concentrations were influenced to a varying degree by fluctuations in flow. We found the lowest output in submandibular/sublingual saliva stimulated with 0.5 % citric acid (3.56 ng/min) and highest output in chewing-stimulated whole saliva (267.01 ng/min). Conclusion. This study shows that saliva contains authentic TIMP-1, the concentration of which was found to depend on gland type and salivary flow. Stimulated whole saliva is suggested as a reliable and easily accessible source for TIMP-1 determinations in bodily fluids.
U2 - 10.1080/00365510701883180
DO - 10.1080/00365510701883180
M3 - Journal article
C2 - 18609089
SN - 0036-5513
VL - 68
SP - 548
EP - 554
JO - Scandinavian Journal of Clinical & Laboratory Investigation
JF - Scandinavian Journal of Clinical & Laboratory Investigation
IS - 7
ER -
