Abstract
DNA sequence analysis has revealed that an unidentified open reading frame (ufr1) is present immediately preceding the aroB gene of Escherichia coli. The predicted protein product of urf1 contains a consensus ATP-binding-site sequence and shows 34% amino acid homology to shikimate kinase II in a 97-amino-acid region. Inactivation of urf1 by insertion of an antibiotic resistance gene had a polar effect on aroB, indicating that these two genes constitute a transcriptional unit. The auxotrophic requirements of a strain mutant for both urf1 and aroL (encoding shikimate kinase II) are consistent with shikimate kinase deficiency. We propose that urf1 encodes shikimate kinase I and that it be designated aroK.
| Original language | English |
|---|---|
| Journal | Journal of Bacteriology |
| Volume | 174 |
| Issue number | 2 |
| Pages (from-to) | 525-9 |
| Number of pages | 5 |
| ISSN | 0021-9193 |
| Publication status | Published - Jan 1992 |
Keywords
- Amino Acid Sequence
- Base Sequence
- Chromosomes, Bacterial
- Cloning, Molecular
- DNA Transposable Elements
- Escherichia coli/enzymology
- Genes, Bacterial
- Lac Operon
- Molecular Sequence Data
- Mutation
- Open Reading Frames
- Phosphotransferases/genetics
- Phosphotransferases (Alcohol Group Acceptor)
- Transcription, Genetic