Identification of the gene (aroK) encoding shikimic acid kinase I of Escherichia coli

A Løbner-Olesen; M G Marinus

Identification of the gene (aroK) encoding shikimic acid kinase I of Escherichia coli

Functional genomics

43 Citationer (Scopus)

Abstract

DNA sequence analysis has revealed that an unidentified open reading frame (ufr1) is present immediately preceding the aroB gene of Escherichia coli. The predicted protein product of urf1 contains a consensus ATP-binding-site sequence and shows 34% amino acid homology to shikimate kinase II in a 97-amino-acid region. Inactivation of urf1 by insertion of an antibiotic resistance gene had a polar effect on aroB, indicating that these two genes constitute a transcriptional unit. The auxotrophic requirements of a strain mutant for both urf1 and aroL (encoding shikimate kinase II) are consistent with shikimate kinase deficiency. We propose that urf1 encodes shikimate kinase I and that it be designated aroK.

Originalsprog	Engelsk
Tidsskrift	Journal of Bacteriology
Vol/bind	174
Udgave nummer	2
Sider (fra-til)	525-9
Antal sider	5
ISSN	0021-9193
Status	Udgivet - jan. 1992

Citationsformater

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title = "Identification of the gene (aroK) encoding shikimic acid kinase I of Escherichia coli",

abstract = "DNA sequence analysis has revealed that an unidentified open reading frame (ufr1) is present immediately preceding the aroB gene of Escherichia coli. The predicted protein product of urf1 contains a consensus ATP-binding-site sequence and shows 34% amino acid homology to shikimate kinase II in a 97-amino-acid region. Inactivation of urf1 by insertion of an antibiotic resistance gene had a polar effect on aroB, indicating that these two genes constitute a transcriptional unit. The auxotrophic requirements of a strain mutant for both urf1 and aroL (encoding shikimate kinase II) are consistent with shikimate kinase deficiency. We propose that urf1 encodes shikimate kinase I and that it be designated aroK.",

keywords = "Amino Acid Sequence, Base Sequence, Chromosomes, Bacterial, Cloning, Molecular, DNA Transposable Elements, Escherichia coli/enzymology, Genes, Bacterial, Lac Operon, Molecular Sequence Data, Mutation, Open Reading Frames, Phosphotransferases/genetics, Phosphotransferases (Alcohol Group Acceptor), Transcription, Genetic",

author = "A L{\o}bner-Olesen and Marinus, {M G}",

year = "1992",

month = jan,

language = "English",

volume = "174",

pages = "525--9",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

number = "2",

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TY - JOUR

T1 - Identification of the gene (aroK) encoding shikimic acid kinase I of Escherichia coli

AU - Løbner-Olesen, A

AU - Marinus, M G

PY - 1992/1

Y1 - 1992/1

N2 - DNA sequence analysis has revealed that an unidentified open reading frame (ufr1) is present immediately preceding the aroB gene of Escherichia coli. The predicted protein product of urf1 contains a consensus ATP-binding-site sequence and shows 34% amino acid homology to shikimate kinase II in a 97-amino-acid region. Inactivation of urf1 by insertion of an antibiotic resistance gene had a polar effect on aroB, indicating that these two genes constitute a transcriptional unit. The auxotrophic requirements of a strain mutant for both urf1 and aroL (encoding shikimate kinase II) are consistent with shikimate kinase deficiency. We propose that urf1 encodes shikimate kinase I and that it be designated aroK.

AB - DNA sequence analysis has revealed that an unidentified open reading frame (ufr1) is present immediately preceding the aroB gene of Escherichia coli. The predicted protein product of urf1 contains a consensus ATP-binding-site sequence and shows 34% amino acid homology to shikimate kinase II in a 97-amino-acid region. Inactivation of urf1 by insertion of an antibiotic resistance gene had a polar effect on aroB, indicating that these two genes constitute a transcriptional unit. The auxotrophic requirements of a strain mutant for both urf1 and aroL (encoding shikimate kinase II) are consistent with shikimate kinase deficiency. We propose that urf1 encodes shikimate kinase I and that it be designated aroK.

KW - Amino Acid Sequence

KW - Base Sequence

KW - Chromosomes, Bacterial

KW - Cloning, Molecular

KW - DNA Transposable Elements

KW - Escherichia coli/enzymology

KW - Genes, Bacterial

KW - Lac Operon

KW - Molecular Sequence Data

KW - Mutation

KW - Open Reading Frames

KW - Phosphotransferases/genetics

KW - Phosphotransferases (Alcohol Group Acceptor)

KW - Transcription, Genetic

M3 - Journal article

C2 - 1309529

SN - 0021-9193

VL - 174

SP - 525

EP - 529

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 2

ER -

Identification of the gene (aroK) encoding shikimic acid kinase I of Escherichia coli

Abstract

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