Identification of peptide and protein doping related drug compounds confiscated in Denmark between 2007-2013

Rune Andersen Hartvig; Niels Bjerre Holm; Petur Weihe Dalsgaard; Lotte Ask Reitzel; Irene Breum Müller; Kristian Linnet

doi:10.2478/sjfs-2014-0003

Identification of peptide and protein doping related drug compounds confiscated in Denmark between 2007-2013

Rune Andersen Hartvig, Niels Bjerre Holm, Petur Weihe Dalsgaard, Lotte Ask Reitzel, Irene Breum Müller, Kristian Linnet

Section of Forensic Chemistry

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Abstract

We present an overview of protein and peptide compounds confiscated in Denmark from late 2007 till late 2013 together with a
description of a newly developed HRAM-LC-MS method used for identification. As examples of identification, we present data for the
peptides AOD-9604, [D-Ala2, Gln8, Ala15, Leu27]sermorelin and the protein follistatin. It was found that a method with minimum sample
preparation could be implemented for all of the confiscated peptides and the protein somatropin. However, for the protein follistatin it
was necessary to include trypsin digestion in the sample preparation, which considerably increases the overall analysis time.

Original language	English
Journal	Scandinavian Journal of Forensic Science
Volume	20
Issue number	2
Pages (from-to)	42-49
Number of pages	8
ISSN	1503-9552
DOIs	https://doi.org/10.2478/sjfs-2014-0003
Publication status	Published - Dec 2014

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10.2478/sjfs-2014-0003Licence: CC BY-NC-ND

Identification of peptide and protein doping related drug compounds confiscated in Denmark between 2007-2013Final published version, 407 KBLicence: CC BY-NC-ND

Cite this

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title = "Identification of peptide and protein doping related drug compounds confiscated in Denmark between 2007-2013",

abstract = "We present an overview of protein and peptide compounds confiscated in Denmark from late 2007 till late 2013 together with adescription of a newly developed HRAM-LC-MS method used for identification. As examples of identification, we present data for thepeptides AOD-9604, [D-Ala2, Gln8, Ala15, Leu27]sermorelin and the protein follistatin. It was found that a method with minimum samplepreparation could be implemented for all of the confiscated peptides and the protein somatropin. However, for the protein follistatin itwas necessary to include trypsin digestion in the sample preparation, which considerably increases the overall analysis time.",

author = "Hartvig, {Rune Andersen} and Holm, {Niels Bjerre} and Dalsgaard, {Petur Weihe} and Reitzel, {Lotte Ask} and M{\"u}ller, {Irene Breum} and Kristian Linnet",

year = "2014",

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language = "English",

volume = "20",

pages = "42--49",

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T1 - Identification of peptide and protein doping related drug compounds confiscated in Denmark between 2007-2013

AU - Hartvig, Rune Andersen

AU - Holm, Niels Bjerre

AU - Dalsgaard, Petur Weihe

AU - Reitzel, Lotte Ask

AU - Müller, Irene Breum

AU - Linnet, Kristian

PY - 2014/12

Y1 - 2014/12

N2 - We present an overview of protein and peptide compounds confiscated in Denmark from late 2007 till late 2013 together with adescription of a newly developed HRAM-LC-MS method used for identification. As examples of identification, we present data for thepeptides AOD-9604, [D-Ala2, Gln8, Ala15, Leu27]sermorelin and the protein follistatin. It was found that a method with minimum samplepreparation could be implemented for all of the confiscated peptides and the protein somatropin. However, for the protein follistatin itwas necessary to include trypsin digestion in the sample preparation, which considerably increases the overall analysis time.

AB - We present an overview of protein and peptide compounds confiscated in Denmark from late 2007 till late 2013 together with adescription of a newly developed HRAM-LC-MS method used for identification. As examples of identification, we present data for thepeptides AOD-9604, [D-Ala2, Gln8, Ala15, Leu27]sermorelin and the protein follistatin. It was found that a method with minimum samplepreparation could be implemented for all of the confiscated peptides and the protein somatropin. However, for the protein follistatin itwas necessary to include trypsin digestion in the sample preparation, which considerably increases the overall analysis time.

U2 - 10.2478/sjfs-2014-0003

DO - 10.2478/sjfs-2014-0003

M3 - Journal article

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VL - 20

SP - 42

EP - 49

JO - Scandinavian Journal of Forensic Science

JF - Scandinavian Journal of Forensic Science

IS - 2

ER -