Identification of peptide and protein doping related drug compounds confiscated in Denmark between 2007-2013

Rune Andersen Hartvig; Niels Bjerre Holm; Petur Weihe Dalsgaard; Lotte Ask Reitzel; Irene Breum Müller; Kristian Linnet

doi:10.2478/sjfs-2014-0003

Identification of peptide and protein doping related drug compounds confiscated in Denmark between 2007-2013

Rune Andersen Hartvig, Niels Bjerre Holm, Petur Weihe Dalsgaard, Lotte Ask Reitzel, Irene Breum Müller, Kristian Linnet

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Abstract

We present an overview of protein and peptide compounds confiscated in Denmark from late 2007 till late 2013 together with a
description of a newly developed HRAM-LC-MS method used for identification. As examples of identification, we present data for the
peptides AOD-9604, [D-Ala2, Gln8, Ala15, Leu27]sermorelin and the protein follistatin. It was found that a method with minimum sample
preparation could be implemented for all of the confiscated peptides and the protein somatropin. However, for the protein follistatin it
was necessary to include trypsin digestion in the sample preparation, which considerably increases the overall analysis time.

Originalsprog	Engelsk
Tidsskrift	Scandinavian Journal of Forensic Science
Vol/bind	20
Udgave nummer	2
Sider (fra-til)	42-49
Antal sider	8
ISSN	1503-9552
DOI	https://doi.org/10.2478/sjfs-2014-0003
Status	Udgivet - dec. 2014

Adgang til dokumentet

10.2478/sjfs-2014-0003Licens: CC BY-NC-ND

Identification of peptide and protein doping related drug compounds confiscated in Denmark between 2007-2013Forlagets udgivne version, 407 KBLicens: CC BY-NC-ND

Citationsformater

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title = "Identification of peptide and protein doping related drug compounds confiscated in Denmark between 2007-2013",

abstract = "We present an overview of protein and peptide compounds confiscated in Denmark from late 2007 till late 2013 together with adescription of a newly developed HRAM-LC-MS method used for identification. As examples of identification, we present data for thepeptides AOD-9604, [D-Ala2, Gln8, Ala15, Leu27]sermorelin and the protein follistatin. It was found that a method with minimum samplepreparation could be implemented for all of the confiscated peptides and the protein somatropin. However, for the protein follistatin itwas necessary to include trypsin digestion in the sample preparation, which considerably increases the overall analysis time.",

author = "Hartvig, {Rune Andersen} and Holm, {Niels Bjerre} and Dalsgaard, {Petur Weihe} and Reitzel, {Lotte Ask} and M{\"u}ller, {Irene Breum} and Kristian Linnet",

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AU - Hartvig, Rune Andersen

AU - Holm, Niels Bjerre

AU - Dalsgaard, Petur Weihe

AU - Reitzel, Lotte Ask

AU - Müller, Irene Breum

AU - Linnet, Kristian

PY - 2014/12

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AB - We present an overview of protein and peptide compounds confiscated in Denmark from late 2007 till late 2013 together with adescription of a newly developed HRAM-LC-MS method used for identification. As examples of identification, we present data for thepeptides AOD-9604, [D-Ala2, Gln8, Ala15, Leu27]sermorelin and the protein follistatin. It was found that a method with minimum samplepreparation could be implemented for all of the confiscated peptides and the protein somatropin. However, for the protein follistatin itwas necessary to include trypsin digestion in the sample preparation, which considerably increases the overall analysis time.

U2 - 10.2478/sjfs-2014-0003

DO - 10.2478/sjfs-2014-0003

M3 - Journal article

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VL - 20

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EP - 49

JO - Scandinavian Journal of Forensic Science

JF - Scandinavian Journal of Forensic Science

IS - 2

ER -