Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells

Sergey V. Anisimov; Nicolaj S. Christophersen; Ana S. Correia; Vanessa Jane Hall; Ingrid Sandeling; Jia-Yi Li; Patrik Brundin

doi:10.2478/s11658-010-0039-8

Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells

Sergey V. Anisimov, Nicolaj S. Christophersen, Ana S. Correia, Vanessa Jane Hall, Ingrid Sandeling, Jia-Yi Li, Patrik Brundin

17 Citations (Scopus)

Abstract

The majority of human embryonic stem cell lines depend on a feeder cell layer for continuous growth in vitro, so that they can remain in an undifferentiated state. Limited knowledge is available concerning the molecular mechanisms that underlie the capacity of feeder cells to support both the proliferation and pluripotency of these cells. Importantly, feeder cells generally lose their capacity to support human embryonic stem cell proliferation in vitro following long-term culture. In this study, we performed large-scale gene expression profiles of human foreskin fibroblasts during early, intermediate and late passages using a custom DNA microarray platform (NeuroStem 2.0 Chip). The microarray data was validated using RT-PCR and virtual SAGE analysis. Our comparative gene expression study identified a limited number of molecular targets potentially involved in the ability of human neonatal foreskin fibroblasts to serve as feeder cells for human embryonic stem cell cultures. Among these, the C-KIT, leptin and pigment epithelium-derived factor (PEDF) genes were the most interesting candidates.

Original language	English
Journal	Cellular & Molecular Biology Letters
Volume	16
Issue number	1
Pages (from-to)	79-88
Number of pages	10
ISSN	1425-8153
DOIs	https://doi.org/10.2478/s11658-010-0039-8
Publication status	Published - Mar 2011

Keywords

Former LIFE faculty

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10.2478/s11658-010-0039-8

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Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells. / Anisimov, Sergey V.; Christophersen, Nicolaj S.; Correia, Ana S. et al.
In: Cellular & Molecular Biology Letters, Vol. 16, No. 1, 03.2011, p. 79-88.

Research output: Contribution to journal › Journal article › Research › peer-review

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abstract = "The majority of human embryonic stem cell lines depend on a feeder cell layer for continuous growth in vitro, so that they can remain in an undifferentiated state. Limited knowledge is available concerning the molecular mechanisms that underlie the capacity of feeder cells to support both the proliferation and pluripotency of these cells. Importantly, feeder cells generally lose their capacity to support human embryonic stem cell proliferation in vitro following long-term culture. In this study, we performed large-scale gene expression profiles of human foreskin fibroblasts during early, intermediate and late passages using a custom DNA microarray platform (NeuroStem 2.0 Chip). The microarray data was validated using RT-PCR and virtual SAGE analysis. Our comparative gene expression study identified a limited number of molecular targets potentially involved in the ability of human neonatal foreskin fibroblasts to serve as feeder cells for human embryonic stem cell cultures. Among these, the C-KIT, leptin and pigment epithelium-derived factor (PEDF) genes were the most interesting candidates.",

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AU - Sandeling, Ingrid

AU - Li, Jia-Yi

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AB - The majority of human embryonic stem cell lines depend on a feeder cell layer for continuous growth in vitro, so that they can remain in an undifferentiated state. Limited knowledge is available concerning the molecular mechanisms that underlie the capacity of feeder cells to support both the proliferation and pluripotency of these cells. Importantly, feeder cells generally lose their capacity to support human embryonic stem cell proliferation in vitro following long-term culture. In this study, we performed large-scale gene expression profiles of human foreskin fibroblasts during early, intermediate and late passages using a custom DNA microarray platform (NeuroStem 2.0 Chip). The microarray data was validated using RT-PCR and virtual SAGE analysis. Our comparative gene expression study identified a limited number of molecular targets potentially involved in the ability of human neonatal foreskin fibroblasts to serve as feeder cells for human embryonic stem cell cultures. Among these, the C-KIT, leptin and pigment epithelium-derived factor (PEDF) genes were the most interesting candidates.

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